, Volume 44, Issue 10, pp 882–885 | Cite as

Inhibitory effect of flavonoids on DNA-dependent DNA and RNA polymerases

  • K. Shinozuka
  • Y. Kikuchi
  • C. Nishino
  • A. Mori
  • S. Tawata
Short Communications


Flavonoids, (−)-epigallocatechin (1), myricetin (2) and quercetin (3), were investigated for inhibitory effects onE. coli DNA polymerase I and T7 bacteriophage RNA polymerase. In both DNA and RNA synthesis,1 and3 inhibited enzyme reactions by non-competitive and mixed type inhibition respecitively, with regard to template DNAs. Myricetin (2) inhibited DNA and RNA polymerase reactions by mixed type and competitive type inhibition, respectively, with template DNAs. It was suggested that2 interacts with covalently closed/circular DNA.

Key words

Flavonoids DNA polymerase RNA polymerase inhibitory effect 


  1. 1.
    Havsteen, B., Biochem. Pharmac.32 (1983) 1141.Google Scholar
  2. 2.
    Nishino, C., Enoki, N., Tawata, S., Mori, A., Kobayashi, K., and Fukushima, M., Agric. biol. Chem.5 (1987) 139.Google Scholar
  3. 3.
    Mori, A., Nishino, C., Enoki, N., and Tawata, S., Phytochemistry26 (1987) 2231.Google Scholar
  4. 4.
    Mori, A., Nishino, C., Enoki, N., and Tawata, S., Phytochemistry27 (1988) 1017.Google Scholar
  5. 5.
    Setlow, P., in: Method of Enzymology, vol. 29, p. 3. Eds L. Grossman and K. Moldave. Academic Press, New York 1974.Google Scholar
  6. 6.
    Schetters, H., Gassen, H. G., and Matthaei, H., Biochem. biophys. Acta272 (1972) 547.Google Scholar
  7. 7.
    Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., and Green, M. R., Nucl. Acids Res.12 (1984) 7035.Google Scholar
  8. 8.
    Müller, W., and Crothers, D. M., J. molec. Biol.35 (1986) 251.Google Scholar
  9. 9.
    Beretz, A., Anton, R., and Stoclet, J. C., Experientia34 (1987) 1054.Google Scholar
  10. 10.
    Chrisey, L. A., Bonjar, G. H. S., and Hecht, S. M., J. Am. chem. Soc.110 (1988) 644.Google Scholar
  11. 11.
    Nose, K., Biochem. Pharmac.33 (1984) 3823.Google Scholar
  12. 12.
    Hardigree, A. A., and Epler, J. L., Mutat. Res.58 (1978) 231.Google Scholar

Copyright information

© Birkhäuser Verlag Basel 1988

Authors and Affiliations

  • K. Shinozuka
    • 1
  • Y. Kikuchi
    • 1
  • C. Nishino
    • 1
  • A. Mori
    • 1
  • S. Tawata
    • 2
  1. 1.Mitsubishi Kasei Institute of Life SciencesMachida, Tokyo(Japan)
  2. 2.Department of Agricultural ChemistryUniversity of RyukyusNishihara, Okinawa(Japan)

Personalised recommendations