The Journal of Membrane Biology

, Volume 37, Issue 1, pp 29–43 | Cite as

Cyclic nucleotide phosphodiesterases of the renal cortex

Characterization of basal-lateral membrane activities
  • Charles R. Filburn
  • C. Tony Liang
  • Bertram Sacktor
Article
Received:

Summary

Cyclic nucleotide phosphodiesterase in the basal-lateral segment of plasma membranes from proximal tubule cells of the rabbit renal cortex was studied and compared to that in the brush border segment of the plasma membrane. Both adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate were hydrolyzed by the basal-lateral membrane, but activity varied differently with the two substrates in a complex concentration-dependent manner. Activity with adenosine 3′,5′-monophosphate was greater than, equal to, or less than with guanosine 3′,5′-monophosphate, at concentrations of 1000, 100, and 10 to 1 μm, respectively. Basal-lateral membrane phosphodiesterase activities at 1 and 500 μm substrate exhibited differential responses to pH, metals, heat, and a heat stable inhibitor. Stimulation by guanosine 3′,5′-monophosphate and inosine 3′,5′-monophosphate of adenosine 3′,5′-monophosphate hydrolysis was found in basal-lateral but not in brush border membranes. This stimulation was potentiated by ethyleneglycol-bis(β-aminoethyl ether)N,N′-tetraacetic acid and ethylenediaminetetraacetate, inhibited by Triton X-100, and totally blocked by Zn2+. The findings indicate that multiple forms of phosphodiesterase are present in the basal-lateral segment and these differ from the activities in the brush border region of the plasma membrane. The characteristics of (i) allosteric, guanosine 3′,5′-monophosphate-sensitivity of adensoine 3′,5′-monophosphate phosphodiesterase, and (ii) relatively high guanosine 3′,5′-monophosphate phosphodiesterase activity, in basal-lateral membranes, which are also enriched in adenylate and guanylate cyclase, suggest an important physiological role for these phosphodiesterases in the regulation of net production of cyclic nucleotides in the renal cortex.

Keywords

Monophosphate Guanosine Phosphodiesterase Brush Border Renal Cortex 
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Copyright information

© Springer-Verlag New York Inc 1977

Authors and Affiliations

  • Charles R. Filburn
    • 1
  • C. Tony Liang
    • 1
  • Bertram Sacktor
    • 1
  1. 1.Laboratory of Molecular Aging, Gerontology Research Center, National Institute on Aging, National Institutes of HealthBaltimore City HospitalsBaltimore

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