Experientia

, Volume 46, Issue 10, pp 1002–1005

Intercellular dye-coupling in intestinal smooth muscle. Are gap junctions required for intercellular coupling?

  • O. Zamir
  • M. Hanani
Research Articles

Summary

The dye Lucifer Yellow was injected into single smooth muscle cells in the guinea pig small intestine in order to study intercellular coupling. Dye-coupling was observed in both the circular and longitudinal muscle layers and was markedly reduced when the intercellular pH was lowered. These results suggest the presence of gap junctions among intestinal muscle cells, but are inconsistent with previous ultrastructural studies that failed to demonstrate such junctions in the longitudinal muscle.

Key words

Gap junctions dye-coupling Lucifer Yellow intestine smooth muscle 

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Literature

  1. 1.
    Beyer, E. C., Kistler, J., Paul, D. L., and Goodenough, D. A., J. Cell Biol.108 (1989) 595.PubMedGoogle Scholar
  2. 2.
    Dewey, M. M., and Barr, L., Science137 (1962) 670.Google Scholar
  3. 3.
    Gabella, G., Br. med. Bull.35 (1979) 213.PubMedGoogle Scholar
  4. 4.
    Daniel E. E., Daniel, V. P., Duchon, G., Garfield, R. E., Nichols, M., Malhorta, S. K., and Oki, M., J. Membr. Biol.28 (1976) 207.PubMedGoogle Scholar
  5. 5.
    Gabella, G., and Blundell, D., Cell Tissue Res.219 (1981) 469.PubMedGoogle Scholar
  6. 6.
    Stewart, W. W., Cell14 (1978) 741.PubMedGoogle Scholar
  7. 7.
    Blennerhassett, M. G., Kannan, M. S., and Garfield, R. E., Am. J. Physiol.252 (1987) C555.PubMedGoogle Scholar
  8. 8.
    Hanani, M., Baluk, P., and Burnstock, G., J. auton. Nerv. System5 (1982) 155.Google Scholar
  9. 9.
    Hanani, M., Chorev, M., Giloin, C., and Selinger, Z., Eur. J. Pharmac.153 (1988) 247.Google Scholar
  10. 10.
    Hanani, M., Zamir, O., and Baluk, P., Brain Res.497 (1989) 245.PubMedGoogle Scholar
  11. 11.
    Spray, D. C., and Bennett, M. V. L., A. Rev. Physiol.47 (1985) 281.Google Scholar
  12. 12.
    Thomas, R. C., J. Physiol. (London)238 (1974) 159.Google Scholar
  13. 13.
    Miyachi, E.-I., and Murakami, M., J. Physiol. (London)419 (1989) 213.Google Scholar
  14. 14.
    Bodmer, R., Verselis, V., Levitan, I. B., and Spray, D. C., J. Neurosci.7 (1988) 1656.Google Scholar
  15. 15.
    Williams, E. H., and DeHaan, R. L., J. Membr. Biol.60 (1981) 237.PubMedGoogle Scholar
  16. 16.
    Connor, J. A., Kreulen, D., Prosser, C. L., and Weigel, R., J. Physiol. (London)273 (1977) 665.Google Scholar
  17. 17.
    Bortoff, A., Michaels, D., and Mistretta, P., Am. J. Physiol.240 (1981) C135.PubMedGoogle Scholar
  18. 18.
    Szurszewski, J. H., in: Physiology of the Gastrointestinal Tract, p. 382. Ed. L. R. Johnson, Raven Press, New York 1987.Google Scholar

Copyright information

© Birkhäuser Verlag Basel 1990

Authors and Affiliations

  • O. Zamir
    • 1
  • M. Hanani
    • 1
  1. 1.Laboratory of Experimental SurgeryHadassah University HospitalJerusalem(Israel)

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