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Basic Research in Cardiology

, Volume 79, Issue 1, pp 49–58 | Cite as

Subcellular origin of the surface fluorescence of reduced nicotinamide nucleotides in the isolated perfused rat heart

  • E. M. Nuutinen
Original Contributions

Summary

Surface fluorometric measurements and indicator metabolite determinations in the isolated perfused rat heart showed that the NADH+NADPH fluorescence of the intact tissue originates largely from the mitochondria. The redox potential of the lactate dehydrogenase system calculated from the endogenous lactate/pyruvate ratios was closely similar to that of the glycerol-3-phosphate dehydrogenase system calculated from the concentrations of glycerol-3-phosphate and dihydroxyacetone phosphate in the tissue. Thus, in contrast to the liver, the cytosolic redox state of the NADH/NAD+ system in isolated perfused heart oxidizing external glucose or fatty acid is not amenable to optical monitoring, but can be assessed from the state of the lactate dehydrogenase or glycerol-3-phosphate, dehydrogenase systems.

Key words

organ fluorometry pyridine nucleotide fluorescence redox state cytosolic redox indicator reactions liver myocardium 

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1984

Authors and Affiliations

  • E. M. Nuutinen
    • 1
  1. 1.Department of Medical BiochemistryUniversity of OuluOuluFinland

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