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Experientia

, Volume 48, Issue 3, pp 271–272 | Cite as

Transfer of isolated nuclei into protoplasts ofAspergillus nidulans

  • Cs. Vágvölgyi
  • L. Ferenczy
Article

Abstract

Nuclei were isolated from protoplasts of a haploid auxotrophicAspergillus nidulans strain. Transformation of protoplasts prepared from a complementary haploid auxotrophic strain with these purified nuclei resulted in both heterokaryotic and diploid colonies. The nutritionally-complementing colonies appeared at a frequency of 5×10−7 to 10−8.

Key words

Protoplast nuclear transfer heterokaryotic colonies nutritional complementation Aspergillus nidulans 

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Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • Cs. Vágvölgyi
    • 1
  • L. Ferenczy
    • 1
  1. 1.Department of MicrobiologyAttila József UniversitySzeged(Hungary)

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