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Experientia

, Volume 47, Issue 9, pp 968–969 | Cite as

An improved test for Africanized honeybee mitochondrial DNA

  • Y. C. Crozier
  • S. Koulianos
  • R. H. Crozier
Research Articles

Abstract

Mitochondrial DNA derived fromApis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks aBglII restriction site found in other types of honeybee1,2. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplifiel product withBglII yields contrasting patterns between Africanized and other honeybees.

Key words

Africanized honeybee Apis mellifera scutellata mitochondrial DNA restriction fragment length polymorphism BglII polymerase chain reaction 

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • Y. C. Crozier
    • 1
  • S. Koulianos
    • 1
  • R. H. Crozier
    • 1
  1. 1.Department of Genetics & Human VariationLa Trobe UniversityBundooraAustralia

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