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Experientia

, Volume 51, Issue 2, pp 126–132 | Cite as

Effect of temperature on in vitro proliferative activity of human umbilical vein endothelial cells

  • Q. R. Yang
  • D. Vanden Berghe
Article

Abstract

Human umbilical vein endothelial cells, skin fibroblasts, and retinal pigment epithelial cells are cultivated in medium supplemented with 15 to 20% serum in our laboratory. The effects of various incubation temperatures on the proliferation of these cells was examined. Our study shows that the mitogenic response of the endothelial cells to a change of temperature differed markedly from that of the fibroblasts and epithelial cells. Cultivation of human umbilical vein endothelial at 37°C required seeding densities as high as 1–2×104 cells/cm2, and yet resulted in a low growth rate and premature senescence. However, under the same culture conditions, but at 33°C, the proliferative capacity of these endothelial cells was potentiated. The results were striking; at 33°C the cells grew actively and the life span was extended. The number of cumulative population doublings increased fourfold compared with that for the same cells cultivated at 37°C. The inoculum size could be reduced, since at 33°C the endothelial cells were able to replicate at seeding densities as low as 20 cells/cm2. The cells serially subcultured at 33°C retained morphological features and specific immunological markers of endothelial cells.

Key words

Human umbilical vein endothelial cells human retinal pigment epithelial cells human skin fibroblasts incubation temperature cell growth 

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Copyright information

© Birkhäuser Verlag 1995

Authors and Affiliations

  • Q. R. Yang
    • 1
  • D. Vanden Berghe
    • 1
  1. 1.Laboratory of Microbiology and Hygiene, Faculty of Medicine and Pharmaceutical SciencesUniversity of Antwerp (UIA)Wilrijk(Belgium)

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