Experientia

, Volume 32, Issue 9, pp 1125–1126 | Cite as

Purification and partial characterization of lysozyme from mouse small intestine

  • T. L. Peeters
  • G. R. Vantrappen
Specialia Chimica, Biochimica

Summary

Lysozyme was isolated from the small intestine of mice by combined ion-exchange and molecular sieve chromatography. This lysozyme differs from that isolated from the urine of mice with monocytoma in amino acid composition, and migration rate in cellulose acetate electrophoresis. As intestinal lysozyme originates at least in part from the Paneth cell, our results point towards the existence of isozymes of lysozymes in mice.

Keywords

Acetate Cellulose Chromatography Migration Purification 
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References

  1. 2.
    F. J. Morgan andN. Arnheim, in:Lysozyme (Eds.F. F. Osserman, R. E. Canfield andS. Beychok; Academic Press, New York 1973), p. 81.Google Scholar
  2. 4.
    N. Arnheim, inLysozyme (Eds.E. F. Osserman, R. E. Canfield andS. Beychok; Academic Press, New York 1973), p. 153.Google Scholar
  3. 5.
    A. Mouton andJ. Jolles, FEBS Lett.4, 337 (1969).PubMedGoogle Scholar
  4. 6.
    E. F. Osserman, New Engl. J. Med.292, 424 (1975).PubMedGoogle Scholar
  5. 7.
    R. J. Deckx, G. R. Vantrappen andM. M. Parein, Biochim. biophys. Acta139, 204 (1967).PubMedGoogle Scholar
  6. 8.
    Y. Ghoos andG. Vantrappen, Histochem. J.3, 175 (1971).PubMedGoogle Scholar
  7. 9.
    T. L. Peeters andG. R. Vantrappen, Gut16, 553 (1975).PubMedGoogle Scholar
  8. 10.
    S. L. Erlandsen, J. A. Parsons andT. D. Taylor, J. Histochem.22, 401 (1974).PubMedGoogle Scholar
  9. 11.
    M. Klockars andE. F. Osserman, J. Histochem. Cytochem.22, 139 (1974).PubMedGoogle Scholar
  10. 12.
    R. J. Riblet andL. A. Herzenberg, Science168, 1595 (1970).PubMedGoogle Scholar
  11. 13.
    G. H. Beaven andE. R. Holiday, Adv. Prot. Chem.7, 319 (1952).Google Scholar
  12. 14.
    E. F. Osserman andD. P. Lawlor, J. exp. Med.124, 921 (1966).PubMedGoogle Scholar
  13. 15.
    D. S. Prieur, H. M. Olsen andD. M. Young, Am. J. Path.77, 283 (1974).PubMedGoogle Scholar
  14. 16.
    T. L. Peeters, K. Geboes andG. R. Vantrappen, New Engl. J. Med.292, 1349 (1975).Google Scholar
  15. 17.
    T. L. Peeters, G. R. Vantrappen andK. Geboes, Gut17, 300 (1976).PubMedGoogle Scholar
  16. 18.
    J. Jolles andP. Jolles, Helv. chim. Acta52, 2671 (1969).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1976

Authors and Affiliations

  • T. L. Peeters
    • 1
  • G. R. Vantrappen
    • 1
  1. 1.Laboratorium voor gastrointestinale fysiopathologie, Department Medische NavorsingKatholieke Universiteit te LeuvenLeuven(Belgium)

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