The Journal of Membrane Biology

, Volume 77, Issue 2, pp 93–99 | Cite as

Axonal microtubules necessary for generation of sodium current in squid giant axons: II. Effect of colchicine upon asymmetrical displacement current

  • Gen Matsumoto
  • Michinori Ichikawa
  • Akira Tasaki
Articles

Summary

Effect of internal colchicine on asymmetrical displacement currents was studied by internally perfusing squid giant axons with a solution containing colchicine. It was found that (1) asymmetrical displacement currents were composed of two parts; colchicine-sensitive and colchicine-resistant; that (2) the colchicine-sensitive part had a definite rising phase while the colchicine-resistant one showed an instantaneous jump, followed by exponential decay; and that (3) the colchicine-sensitive part related to normal Na channels.

Key Words

Na gating currents Na currents colchicine 

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References

  1. Armstrong, C.M., Bezanilla, F. 1974. Charge movement associated with the opening and closing of the activation gates of the Na channels.J. Gen. Physiol. 63:533–552PubMedGoogle Scholar
  2. Armstrong, C.M., Bezanilla, F. 1977. Inactivation of the sodium channel. II. Gating current experiments.J. Gen. Physiol. 70:567–590PubMedGoogle Scholar
  3. Armstrong, C.M., Gilly, W.F. 1979. Fast and slow steps in the activation of sodium channels.J. Gen. Physiol. 74:691–711PubMedGoogle Scholar
  4. Bezanilla, F., Armstrong, C.M. 1974. Gating currents of the sodium channels: Three ways to block them.Science 183:753–754PubMedGoogle Scholar
  5. Bezanilla, F., Armstrong, C.M. 1975. Kinetic properties and inactivation of the gating currents of sodium channels in squid axon.Philos. Trans. R. Soc. London B 270:449–458Google Scholar
  6. Bezanilla, F., Taylor, R.E. 1978. Temperature effects on gating currents in the squid giant axon.Biophys. J. 23:479–484PubMedGoogle Scholar
  7. Bezanilla, F., Taylor, R.E., Fernández, J.M. 1982. Distribution and kinetics of membrane dielectric polarization 1. Long-term inactivation of gating currents.J. Gen. Physiol. 79:21–40PubMedGoogle Scholar
  8. Gilly, W.F., Armstrong, C.M. 1982. Slowing of sodium channel opening kinetics in squid axon by extracellular zinc.J. Gen. Physiol. 79:935–964PubMedGoogle Scholar
  9. Hodgkin, A.L., Huxley, A.F. 1952. A quantitative description of membrane current and its application to conduction and excitation in nerve.J. Physiol. (London) 117:500–544Google Scholar
  10. Katz, G.M., Schwartz, T.L. 1974. Temporal control of voltageclamped membranes: An examination of principles.J. Membrane Biol. 17:275–291Google Scholar
  11. Keynes, R.D., Rojas, E. 1974. Kinetics and steady-state properties of the charged system controlling sodium conductance in the squid giant axon.J. Physiol. (London) 239:393–434Google Scholar
  12. Keynes, R.D., Rojas, E. 1976. The temporal and steady-state relationship between activation of the sodium conductance and movement of the gating particles in the squid axon.J. Physiol. (London) 255:157–189Google Scholar
  13. Kimura, J.E., Meves, H. 1979. The effect of temperature on the asymmetrical charge movement in squid giant axons.J. Physiol. (London) 289:479–500Google Scholar
  14. Matsumoto, G., Ichikawa, M., Tasaki, A., Murofushi, H., Sakai, H. 1984. Axonal microtubules necessary for generation of sodium current in squid giant axons. I. Pharmacological study on sodium current and Restoration of sodium current by microtubule proteins and 260K protein.J. Membrane Biol. 77:77–91Google Scholar
  15. Meves, H. 1974. The effect of holding potential on the asymmetry currents in squid giant axons.J. Physiol. (London) 243:847–867Google Scholar
  16. Meves, H. 1976. The effect of zinc on the late displacement current in squid giant axons.J. Phusiol. (London) 254:787–801Google Scholar
  17. Meves, H., Vogel, W. 1977a. Inactivation of the asymmetrical displacement current in giant axons ofLoligo forbesi.J. Physiol. (London) 267:377–393Google Scholar
  18. Meves, H., Vogel, W. 1977b. Slow recovery of sodium current and gating current from inactivation.J. Physiol. (London) 267:395–410Google Scholar
  19. Seyama, I., Narahashi, T. 1981. Modulation of sodium channels of squid nerve membranes by grayanotoxin I.J. Pharmacol. Exp. Ther. 219:614–624PubMedGoogle Scholar
  20. Wilson, L. 1970. Properties of colchicine binding protein from chick embryo brain. Interaction with vinca alkaloids and podophyllotoxin.Biochemistry 9:4999–5007PubMedGoogle Scholar
  21. Wilson, L., Bamburg, J.R., Mizel, S.B., Grisham, L.M., Creswell, K.M. 1974. Interaction of drugs with microtubule proteins.Fed. Proc. 33:158–166PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Gen Matsumoto
    • 1
  • Michinori Ichikawa
    • 1
  • Akira Tasaki
    • 1
  1. 1.Electrotechnical Laboratory, Tsukuba Science CityIbarakiJapan
  2. 2.Institute of Applied PhysicsThe University of Tsukuba, Tsukuba Science CityIbarakiJapan

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