Experientia

, Volume 48, Issue 1, pp 8–10

An improved thioflavine S method for staining neurofibrillary tangles and senile plaques in Alzheimer's disease

  • R. Guntern
  • C. Bouras
  • P. R. Hof
  • P. G. Vallet
Research Articles

DOI: 10.1007/BF01923594

Cite this article as:
Guntern, R., Bouras, C., Hof, P.R. et al. Experientia (1992) 48: 8. doi:10.1007/BF01923594

Abstract

Large differences are usually observed when standard staining methods for a number of pathological lesions in neurodegenerative disorders are compared. With the modified thioflavine S method presented here (easy and cheap to perform), the morphological appearance of the stained neurofibrillary tangles (NFT) and senile plaques (SP) is greatly improved. Furthermore, the intense contrast between stained lesions and background obtained with this technique permits an accurate automatic quantification of NFT and SP using a computer-assisted image analysis system.

Key words

Senile plaques neurofibrillary tangles Alzheimer's disease thioflavine S 

Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • R. Guntern
    • 1
  • C. Bouras
    • 1
  • P. R. Hof
    • 2
  • P. G. Vallet
    • 1
  1. 1.Dept PsychiatryUniversity of GenevaGeneva(Switzerland)
  2. 2.Fishberg Res.Ctr.Mt Sinai School of MedicineNew York(USA)

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