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Effect of parathion on honey bee larvae rearedin vitro

  • Chr Czoppelt
  • H. Rembold
Article

Abstract

First instar honey bee larvae (0.2–0.4 mg) from outdoor (summer season) and flight-room colonies (winter season) were reared in an incubator on a semisynthetic diet. Parathion was either dissolved in the diet (0.01–10.0 μg/ml) or topically applied (0–0.5 μg) to the 3rd instar larvae. Increasing doses of parathion caused a delay in larval development and prepupal mortality. The half lethal concentration (LC50) of the insecticide, if applied in the diet, was 0.02 and 0.14 μg/larva, respectively, for summer and for winter larvae. The half lethal dose (LD50) after topical application was 0.18 μg parathion/larva. The utility of rearing honey bee larvaein vitro for evaluation of pesticide toxicity is also discussed.

Keywords

Larval Development Instar Larva Parathion Lethal Concentration Pesticide Toxicity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Junge Honigbienenlarven (1. Stadium, 0.2–0.4 mg), die sowohl aus Freiland- (Sommersaison) als aus Flugraumvölkern (Wintersaison) stammten, wurden im Brutschrank auf halbsynthetischem Futtersaft aufgezogen. Parathion wurde entweder im Futtersaft gelöst (0.01–10.0 μg/ml) an Larven verfüttert oder topikal auf Larven des 3. Stadiums (L3) aufgeträufelt (0–0.5 μg/L3). Steigende Dosen des Insektizids bewirkten eine Verzögerung der Larvenentwicklung und Absterben der Vorpuppen. Die halbletale Konzentration (LC50) des Insektizids lag nach oraler Applikation für Sommer- und Winterlarven bei 0.02 bzw. 0.14 μg/Larve. Nach topikaler Behandlung mit Parathion betrug die halbletale Dosis (LD50) 0.18 μg/Larve. Die Vorteile bei derin vitro-Aufzucht zur Ermittlung der Bienengiftigkeit von Pflanzenschutzmitteln für Larven wird diskutiert.

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Copyright information

© Verlag Paul Parey 1988

Authors and Affiliations

  • Chr Czoppelt
    • 1
  • H. Rembold
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried

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