Plant and Soil

, Volume 28, Issue 2, pp 258–267 | Cite as

Interrelations of micro-organisms and mulberry

II. Phyllosphere microflora and nitrogen fixation in leaf and root surfaces
  • V. N. Vasantharajan
  • J. V. Bhat


The mulberry leaves were shown to harbour substantial populations of bacteria, streptomycetes, yeasts, and moulds. Azotobacter and Beijerinckia were observed to contribute to nearly 5 to 10 per cent of the bacterial population. When grown in water culture under sterile conditions, Azotobacter inoculation on the leaf or root surface was found to increase plant growth, dry wt, and nitrogen content of the mulberry. The beneficial effect of Azotobacter was largely influenced by the presence of a carbon source in the plant nutrient solution. The root inoculation in comparison to leaf application was found to confer greater benefits to the growing plant. The presence of carbohydrates and amino acids in the leaf leachates of mulberry was shown. The mutual beneficial nature of the association of the plant and Azotobacter has been brought to light.


Carbohydrate Carbon Source Nitrogen Content Nutrient Solution Plant Nutrient 
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Copyright information

© Martinus Nijhoff 1968

Authors and Affiliations

  • V. N. Vasantharajan
    • 1
  • J. V. Bhat
    • 1
  1. 1.Fermentation Technology LaboratoryIndian Institute of ScienceBangalore

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