European Journal of Plant Pathology

, Volume 102, Issue 4, pp 377–383 | Cite as

Production, characterization and interaction of single-spore isolates ofPlasmodiophora brassicae

  • Roeland E. Voorrips
Research Articles


Out of 164 plants of clubroot-susceptible Chinese cabbage inoculated with single resting spores ofPlasmodiophora brassicae, two plants developed clubroot symptoms. The two single-spore isolates (SSIs) extracted from these plants gave an identical reaction pattern on the European Clubroot Differential set (ECD) and seven doubled-haploid lines (DH-lines). Their reaction pattern differed from that of the original field isolate on four hosts: ECD hosts 06 and 07 were susceptible to the field isolate but resistant to both SSIs, while for DH-lines Bi and Pt the reverse was true. DH-line Pt was significantly less diseased by mixed inocula consisting of the field isolate and SSI-1 than by SSI-1 alone. It was concluded that the SSI-1 pathotype was a minor component of the field isolate, although it was isolated twice. The results also suggest that the alleviating effect of the field isolate in mixed inoculations with SSI-1 on DH-line Pt was due to induced resistance, rather than to competitive interactions.

Key words

Brassica oleracea B. napus clubroot single-spore isolate induced resistance 





doubled haploid line


European Clubroot Differential set


single-spore isolate


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Roeland E. Voorrips
    • 1
  1. 1.Department of Vegetable and Fruit CropsDLO - Centre for Plant Breeding and Reproduction Research (CPRO-DLO)AA WageningenThe Netherlands

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