Advertisement

European Journal of Plant Pathology

, Volume 102, Issue 4, pp 377–383 | Cite as

Production, characterization and interaction of single-spore isolates ofPlasmodiophora brassicae

  • Roeland E. Voorrips
Research Articles

Abstract

Out of 164 plants of clubroot-susceptible Chinese cabbage inoculated with single resting spores ofPlasmodiophora brassicae, two plants developed clubroot symptoms. The two single-spore isolates (SSIs) extracted from these plants gave an identical reaction pattern on the European Clubroot Differential set (ECD) and seven doubled-haploid lines (DH-lines). Their reaction pattern differed from that of the original field isolate on four hosts: ECD hosts 06 and 07 were susceptible to the field isolate but resistant to both SSIs, while for DH-lines Bi and Pt the reverse was true. DH-line Pt was significantly less diseased by mixed inocula consisting of the field isolate and SSI-1 than by SSI-1 alone. It was concluded that the SSI-1 pathotype was a minor component of the field isolate, although it was isolated twice. The results also suggest that the alleviating effect of the field isolate in mixed inoculations with SSI-1 on DH-line Pt was due to induced resistance, rather than to competitive interactions.

Key words

Brassica oleracea B. napus clubroot single-spore isolate induced resistance 

Abbreviations

cv

cultivar

DH-line

doubled haploid line

ECD

European Clubroot Differential set

SSI

single-spore isolate

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baggett JR and Kean D (1985) Clubroot-resistant broccoli breeding lines OSU CR-2 to OSU CR-8. HortScience 20: 784–785Google Scholar
  2. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR and Hobolth LA (1975) Study of physiologic specialization inPlasmodiophora brassicae: proposals for attempted rationalization through an international approach. Transactions of the British Mycological Society 65: 295–303Google Scholar
  3. Buczacki ST (1977) Root infections from single resting spores ofPlasmodiophora brassicae. Transactions of the British Mycological Society 69: 328–329Google Scholar
  4. Chiang MS and CrÊte R (1970) Inheritance of clubroot resistance in cabbage (Brassica oleracea L. var.capitata). Canadian Journal of Genetics and Cytology 12: 253–256Google Scholar
  5. Duijs JG, Voorrips RE, Visser DL and Custers JBM (1989) Microspore culture is successful in most crop types ofBrassica oleracea L. Euphytica 60: 45–55Google Scholar
  6. Haji Tinggal S and Webster J (1981) Technique for single spore infection byPlasmodiophora brassicae. Transactions of the British Mycological Society 76: 187–190Google Scholar
  7. Jones DR, Ingram DS and Dixon GR (1982a) Factors affecting tests for differential pathogenicity in populations ofPlasmodiophora brassicae. Plant Pathology 31: 229–238Google Scholar
  8. Jones DR, Ingram DS and Dixon GR (1982b) Characterization of isolates derived from single resting spores ofPlasmodiophora brassicae and studies of their interaction. Plant Pathology 31: 239–246Google Scholar
  9. Madamanchi NR and Kuć J (1991) Induced systemic resistance in plants. In: Cole GT and Hoch HC (eds) The fungal spore and disease initiation in plants and animals (pp. 347–362) Plenum Press, New YorkGoogle Scholar
  10. Nieuwhof M and Wiering D (1963) Clubroot resistance inBrassica oleracea L. Euphytica 11: 233–239Google Scholar
  11. Schoeller M and Grunewaldt J (1986) Production and characterization of single spore derived lines ofP. brassicae Wor. Cruciferae Newsletter 11: 110–111Google Scholar
  12. Schulte U (1994) Zur genetischen Characterisierung des Erregers der Kohlhernie,Plasmodiophora brassicae Wor. Dissertation, UniversitÄt Hannover, Germany, 147 ppGoogle Scholar
  13. Scott ES (1985) Production and characterization of single-spore isolates ofPlasmodiophora brassicae. Plant Pathology 34: 287–292Google Scholar
  14. Siegel S (1956). Nonparametric statistics for the behavioural sciences. McGraw-Hill, New YorkGoogle Scholar
  15. Tommerup IC and Ingram DS (1971) The life-cycle ofPlasmodiophora brassicae Woron. inBrassica tissue cultures and in intact roots. New Phytologist 70: 327–332Google Scholar
  16. Voorrips RE (1992) Root hair infection byPlasmodiophora brassicae in clubroot-resistant and susceptible genotypes ofBrassica oleracea, B. rapa andB. napus. Netherlands Journal of Plant Pathology 98: 361–368Google Scholar
  17. Voorrips RE and Visser DL (1993) Examination of resistance to clubroot in accessions ofBrassica oleracea using a glasshouse seedling test. Netherlands Journal of Plant Pathology 99: 269–276Google Scholar
  18. Voorrips RE (1996) A one-hit model for the infection of clubrootsusceptible cabbage (Brassica oleracea varcapitata) byPlasmodiophora brassicae at various inoculum densities. European Journal of Plant Pathology 102: 109–114Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Roeland E. Voorrips
    • 1
  1. 1.Department of Vegetable and Fruit CropsDLO - Centre for Plant Breeding and Reproduction Research (CPRO-DLO)AA WageningenThe Netherlands

Personalised recommendations