A carlavirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle virus as a whitefly-transmitted carlavirus
Cowpea mild mottle virus (CMMV) has physicochemical properties typical of carlaviruses, but has remained unclassified due to a number of unusual properties, including no serological cross-reaction with 18 carlaviruses; production of brush-like inclusion bodiesin vivo; and the ability to be transmitted by whiteflies (Bermisia tabaci). In this paper we report the use of a carlavirus specific PCR primer to identify CMMV as a member of the carlavirus group. This is confirmed by nucleotide sequence (958 nucleotides) from the 3′ terminal region of CMMV RNA which contains a partial open reading frame (ORF) having high similarity with the coat proteins of other carlaviruses. The sequence also contains an 11.7K ORF at the 3′ terminus, containing a ‘zinc-finger’ motif which is unique to carlaviruses.
Key wordscarlavirus-specific primer CMMV nucleotide sequence PCR
Unable to display preview. Download preview PDF.
- Adams AN and Barbara DJ (1980) Host range, purification and some properties of hop mosaic virus. Annals of Applied Biology 96: 201–208Google Scholar
- Adams AN and Barbara DJ (1982) Host range, purification and some properties of two carlaviruses from hop (Humulus lupulus): hop latent and American hop latent. Annals of Applied Biology 101: 483–494Google Scholar
- Jeyanandarajah P and Brunt AA (1993) The natural occurrence, transmission, properties and possible affinities of cowpea mild mottle virus. Journal of Phytopathology 137: 148–156Google Scholar
- Pappu SS, Brand R, Pappu HR, Rybicki EP, Gough KH, Frenkel MJ and Niblett CL (1993). A polymerase chain reaction method adapted for selected amplification and cloning of 3′ sequences of potyviral genomes: application to Dasheen mosaic virus. Journal of Virological Methods 41: 9–20PubMedGoogle Scholar
- Shukla, DD, Ward CW and Brunt AA (1994) The Potyviridae. CAB International, Oxon, UKGoogle Scholar