Enhancement of interferon-β production with sphingomyelin from fermented milk
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A fermented milk, Kefir, contains an active substance which enhances IFN-β secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2–3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.
Key wordsInterferon-β Sphingomyelin Sphingosine Fermented milk
fast atom bombardment mass spectrometry
fast atom bombardment tandem mass spectrometry
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