Biotechnology Techniques

, Volume 3, Issue 4, pp 217–222 | Cite as

Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes

  • Sadettin S. Ozturk
  • Mark E. Meyerhoff
  • Bernhard O. Palsson
Article

Summary

This paper describes the use of a commercially available off-line gas sensing electrode for determination of ammonia and glutamine in cell culture media. The measurement technique was tested in different media preparations with different serum concentrations. The glutamine decomposition was studied as a function of pH for cell culture medium and the results were compared to those obtained by conventional methods,i.e., HPLC. Finally, glutamine and ammonia metabolism were studied during the cultivation of a hybridoma cell line.

Keywords

Ammonia Cell Culture HPLC Glutamine Serum Concentration 
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Copyright information

© Science & Technology Letters 1989

Authors and Affiliations

  • Sadettin S. Ozturk
    • 2
  • Mark E. Meyerhoff
    • 1
  • Bernhard O. Palsson
    • 2
  1. 1.Department of ChemistryThe University of MichiganAnn ArborUSA
  2. 2.Department of Chemical EngineeringThe University of MichiganAnn ArborUSA

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