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A novel anaerobic needle inoculator for strict obligate anaerobic bacteria


An inoculating needle for picking and transferring colonies of strict anaerobic bacteria was built. A self-refilling syringe was adapted to carry a stainless steel needle within the cannula, and a tee junction allowed continuous flushing with dioxygen-free gas.

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Kinoshita, M., Paynter, M.J.B. A novel anaerobic needle inoculator for strict obligate anaerobic bacteria. Biotechnol Tech 2, 163–164 (1988).

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  • Stainless Steel
  • Syringe
  • Flushing
  • Anaerobic Bacterium
  • Stainless Steel Needle