An inoculating needle for picking and transferring colonies of strict anaerobic bacteria was built. A self-refilling syringe was adapted to carry a stainless steel needle within the cannula, and a tee junction allowed continuous flushing with dioxygen-free gas.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Bryant, M. P. (1972).Am. J. Clin. Nutr. 25, 1324–1328.
Holdeman, L. V., Cato, E. P., and Moore, W. E. C., eds. (1977).Anaerobe laboratory manual, 4th ed. Blacksburg: Virginia Polytechnic Institute and State University.
Hungate, R. E. (1950).Bacteriol. Rev. 14, 1–49.
Hungate, R. E. (1969). A roll tube method for cultivation of strict anaerobes. In:Methods in microbiology, J. R. Norris and D. W. Ribbons, eds. vol. 3B, pp. 117–132, New York: Academic Press, Inc.
Macy, J. M., Snellen, J. E., and Hungate, R. E. (1972).Am. J. Clin. Nutr. 25, 1318–1323.
About this article
Cite this article
Kinoshita, M., Paynter, M.J.B. A novel anaerobic needle inoculator for strict obligate anaerobic bacteria. Biotechnol Tech 2, 163–164 (1988). https://doi.org/10.1007/BF01875758
- Stainless Steel
- Anaerobic Bacterium
- Stainless Steel Needle