The amylase-producing rat pancreatic tumour cell line AR42J was encapsulated in alginate/poly-L-lysine under conditions where the secreted amylase was retained within the system. On treatment with shock waves from a lithotriptor, the porosity of the capsules was changed and the amylase was released from the system. Conditions of treatment were manipulated in order to maximise release of the amylase without significantly reducing the cell viability.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Bunni, L., McHale, L.M. & McHale, A.P. (1989) Enz. Microb. Technol.11 370–375.
Chaussy, C., Schuller, J., Schmiedt, E., Brandl, H., Jocham, D. and Leidl, B. (1984) Urology,23 59–68.
Jessop, N.W. (1980) In vitro16, 212.
Lim, F. & Sun, J. (1980) Carbohydr. Res.86, 97–104.
McCarthy, U., McHale, A.P. & McHale, M.L. (1988) Biochem. Soc. Trans.17 393–394.
McCleary (1980) Carbohydr. Res.86 97–104.
McHale, A.P., McHale, M.L. & Blau, W. (1988) Cancer Biochem. Biophys.10 157–164.
Robert, A., Meyer, S. & Rochner, T. (1986) Ann. Surgery203 286–296.
Valerio, D., Whyte, E.H., Schlam, W.A. & Blackburn, G.L. (1977) J. Parenter. Enteral Nutr.1, 110–114.
Vallancien, G., Aviles, J., Munoz, R., Veillon, B., Charton, M. & Brisset, J.M. (1988) J. Urology139 689–694.
About this article
Cite this article
Jones, B., McCarthy, U., Bunni, L. et al. Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J. Biotechnol Tech 3, 355–360 (1989). https://doi.org/10.1007/BF01875636
- Tumour Cell
- Cell Viability
- Shock Wave