Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Theoretical NMR study of the chemical exchange of amide protons of proteins

Summary

A model is proposed to evaluate the rate of exchange between the amide protons of proteins and the solvent water molecules. Using this model we determined the extent of the error for the chemical exchange rate constant when cross relaxation was neglected; both selective inversion and saturation-transfer techniques were evaluated. Furthermore, the fluctuations in the NOE intensities were determined when the exchange rate was varied.

This is a preview of subscription content, log in to check access.

References

  1. Bloch, F. (1957)Phys. Rev.,105, 1206–1222.

  2. Dill, K., Huang, L., Bearden, D.W., McGown, E.L. and O'Connor, R. (1991)Chem. Res. Toxicol.,4, 295–299.

  3. Englander, S.W., Donner, N.N. and Teitelobaum, H.A. (1972)Annu. Rev. Biochem.,41, 903–924.

  4. Englander, S.W. and Kallenbach, N.R.Q. (1984)Annu. Rev. Biophys.,16, 521–655.

  5. Heatley, F. (1986) inAnnual Reports on NMR Spectroscopy (Ed., Webb, G.A.), Vol. 17, Academic Press, New York, pp. 179–230.

  6. Henry, G.D. and Sykes, B.D. (1990)Biochemistry,29, 6303–6313.

  7. Kaptein, R., Boelens, R., Scheek, R.M. and van Gunsteren, W.F. (1988)Biochemistry,27, 5390–5394.

  8. Led, J.J., Gesmar, H. and Abildgaard, F. (1989)Methods Enzymol.,176, 311–329.

  9. Landy, S.B. and Rao, B.D.N. (1989)J. Magn. Reson.,81, 371–377.

  10. Linderstrom-Lang, K.U. and Schallman, J.A. (1959)Enzymes, 2nd ed., pp. 443–510.

  11. Niccolai, N. and Rossi, C. (1989)Methods Enzymol.,176, 184–199.

  12. Udgaonkar, J.B. and Baldwin, R.L. (1988)Nature,335, 694–699.

  13. Woodward, C., Simon, I. and Tuchsen, E. (1982)Mol. Cell. Biochem.,48, 135–160.

  14. Wright, P.E. (1989)TIBS,14, 255–260.

Download references

Author information

Correspondence to Kilian Dill.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dill, K., Huang, L. & Bearden, D.W. Theoretical NMR study of the chemical exchange of amide protons of proteins. J Biomol NMR 2, 173–181 (1992). https://doi.org/10.1007/BF01875528

Download citation

Keywords

  • Amide proton
  • Exchange rate
  • Kinetics
  • NOE
  • Cross-relaxation