European Journal of Plant Pathology

, Volume 101, Issue 6, pp 627–637

Effect of interrupted leaf wetness periods on suppression of sporulation ofBotrytis allii andB. cinerea by antagonists on dead onion leaves

  • Jürgen Köhl
  • Carin H. van der Plas
  • Wilma M. L. Molhoek
  • Nyckle J. Fokkema
Research Articles

Abstract

Saprophytic antagonists were evaluated for suppression of sporulation ofBotrytis allii andB. cinerea on artificially killed segments of onion leaves that were pre-inoculated with the pathogens. During incubation of the antagonisttreated leaf segments in moist chambers, periods of leaf wetness and leaf dryness were alternated to simulate conditions in the field. Interruption of humid conditions with dry periods had a differential effect on antagonists.Alternaria alternata, Chaetomium globosum, Ulocladium atrum andU. chartarum suppressed sporulation ofB. allii almost completely under continuously wet conditions, and when the leaf wetness periods were interrupted with drying periods of 9h imposed 16, 40, and 64 h after the antagonists were applied. When leaf wetness was interrupted 16 h after antagonist application, the number of conidia ofB. allii produced cm−2 leaf surface after eight days was under the detection limit of 5.2 × 103 conidia on leaves treated with these antagonists compared to 3.7 × 105 conidia on leaves that were not treated. On the other hand,Gliocladium roseum, G. catenulatum andSesquicillium candelabrum, all highly efficient under continuously wet conditions, were of low to moderate efficiency when leaf wetness periods had been interrupted 16 h after application of the antagonists. The antagonists showed the same differentiation and sensitivity to interrupted wetness periods when tested withB. cinerea.

Key words

biological control Botrytis allii Botrytis cinerea leaf wetness onion plant debris 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Jürgen Köhl
    • 1
  • Carin H. van der Plas
    • 1
  • Wilma M. L. Molhoek
    • 1
  • Nyckle J. Fokkema
    • 1
  1. 1.DLO-Research Institute for Plant Protection (IPO-DLO)WageningenThe Netherlands

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