Intracellular microelectrode techniques were employed to study the mechanism by which amphotericin B induces a transient mucosa-negative transepithelial potential (ΔV ms) in the gallbladder ofNecturus. When the tissue was incubated in standard Na-Ringer's solution, the antibiotic reduced the apical membrane potential by about 40 mV, and the basolateral membrane potential by about 35 mV whereas the transepithelial potential increased by about 5 mV. The electrical resistance of the apical membrane fell by 83%, and that of the basolateral membrane by 40%; the paracellular resistance remained unchanged. Circuit analysis indicated that the equivalent electromotive forces of the apical and basolateral membranes fell by 35 and 11 mV, respectively. Changes in potentials and resistances produced by ionic substitutions in the mucosal bathing medium showed that amphotericin B produces a nonselective increase in apical membrane small monovalent cation conductance (K, Na, Li). In the presence of Na-Ringer's on the mucosal side, this resulted in a reduction of the K permselectivity of the membrane, and thus in a fall of its equivalent emf. During short term exposure to amphotericin B,P Na/P Cl across the paracellular pathway did not change significantly, whereasP K/P Na doubled. These results indicate that ΔV ms is due to an increase of gNa across the luminal membranes of the epithelial cells (Cremaschiet al., 1977,J. Membrane Biol. 34:55); the data do not support the alternative hypothesis (Rose & Nahrwold, 1976.J. Membrane Biol. 29:1) that ΔV ms results from a reduction in shuntP Na/P Cl acting in combination with a rheogenic basolateral Na pump.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Barry, P.H., Diamond, J.M. 1970. Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranes.J. Membrane Biol. 3: 93
Barry, P.H., Diamond, J.M., Wright, E.M. 1971. The mechanism of cation permeation in rabbit gallbladder. Dilution potentials and biionic potentials.J. Membrane Biol. 4: 358
Bentley, P.J. 1968. Action of amphotericin B on the toad bladder: Evidence for sodium transport along two pathways.J. Physiol (London) 196: 703
Candia, O.A., Bentley, P.J., Cook, P.I. 1974. Stimulation by amphotericin B of active Na transport across amphibian cornea.Am. J. Physiol. 226: 1438
Cremaschi, D., Hénin, S., Meyer, G., Bacciola, T. 1977. Does amphotericin B unmask an electrogenic Na+ pump in rabbit gallbladder? Shift of gallbladders with negative to gallbladders with positive transepithelial p.d.'s.J. Membrane Biol. 34: 55
Cremaschi, D., Montanari, C., Simonic, T., Lippe, C. 1971. Cholesterol in plasma membranes of rabbit gallbladder epithelium tested with Amphotericin B.Arch. Int. Physiol. Biochim. 79: 33
Frömter, E. 1972. The route of passive ion movement through the epithelium ofNecturus gallbladder.J. Membrane Biol. 8: 259
Hénin, S., Cremaschi, D. 1975. Transcellular ion route in rabbit gallbladder. Electrical properties of the epithelial cells.Pfluegers Arch. 355: 125
Hénin, S., Cremaschi, D., Schettino, T., Meyer, G., Donin, C.L.L., Cotelli, F. 1977. Electrical parameters in gallbladders of different species. Their contribution to the origin of the transmural potential difference.J Membrane Biol. 34: 73
lewis, S.A., Eaton, D.C., Clausen, C., Diamond, J.M. 1977. Nystatin as a probe for investigating the electrical properties of a tight epithelium.J. Gen. Physiol. 70: 427
Machen, T.E., Diamond, J.M. 1969. An estimate of the salt concentration in the lateral intercellular spaces of rabbit gallbladder during maximal fluid transport.J. Membrane Biol. 1: 194
Nielsen, R. 1971. Effect of amphotericin B on frog skinin vitro. Evidence for outward active potassium transport, across the epithelium.Acta Physiol. Scand. 83: 106
Os, G.H. van, Slegers, J.F.G. 1975. The electrical potential profile of gallbladder epithelium.J. Membrane Biol. 24: 341
Reuss, L., Finn, A.L., 1974. Passive electrical properties of toad urinary bladder epithelium. Intercellular electrical coupling and transepithelial cellular and shunt conductances.J. Gen. Physiol. 64: 1
Reuss, L., Finn, A.L. 1975a. Electrical properties of the cellular transepithelial pathway inNecturus gallbladder. I. Circuit analysis and steady-state effects of mucosal solution ionic substitutions.J. Membrane Biol. 25: 115
Reuss, L., Finn, A.L. 1975b. Electrical properties of the cellular transepithelial, pathway inNecturus gallbladder. II. Ionic permeability of the apical cell membrane.J. Membrane Biol. 25: 141
Reuss, L., Finn, A.L. 1977a. Effects of luminal hyperosmolality on electrical pathways ofNecturus gallbladder.Am. J. Physiol. Cell Physiol. 1: C99
Reuss, L., Finn, A.L. 1977b. Mechanisms of voltage transients during current clamp inNecturus gallbladder.J. Membrane Biol. 37: 299
Rose, R. C., Nahrwold, D.L. 1976. Electrolyte transport by gallbladders of rabbit and guinea pig: Effect of amphotericin B and evidence of rheogenic Na transport.J. Membrane Biol. 29: 1
Stroup, R.F., Weinman, E., Hayslett, J.P., Kashgarian, M. 1974. Effect of luminal permeability on net transport across the amphibian proximal tubule.Am. J. Physiol. 226: 1110
About this article
Cite this article
Reuss, L. Effects of amphotericin B on the electrical properties ofNecturus gallbladder: Intracellular microelectrode studies. J. Membrain Biol. 41, 65–86 (1978). https://doi.org/10.1007/BF01873340
- amphotericin B
- leaky epithelia
- sodium transport
- membrane permeability