The Journal of Membrane Biology

, Volume 106, Issue 3, pp 203–210 | Cite as

Growth factors modulate junctional cell-to-cell communication

  • Pedro E. Maldonado
  • Birgit Rose
  • Werner R. Loewenstein


The epidermal growth factor (EGF) and the platelet-derived growth factor (PDGF) inhibit gap junctional communication in the mammalian cell lines NRK and BalbC 3T3: cell-to-cell transfer of a 400-dalton tracer molecule is reduced and junctional conductance is reduced. The inhibition of cell-to-cell transfer is reversible and dose dependent; half-maximal effects are obtained at 10−9 and 10−11m concentrations of EGF and PDGF, respectively. The response of junctional conductance is detectable within 2 min of EGF application and reaches a maximum within 10 min. It is among the earliest cellular responses to this growth factor and may be significant in the regulation of growth. The response is lacking in EGF receptor-deficient NIH 3T3 cells. The transforming factor β (TGFβ) enhances junctional communication in BalbC 3T3: cell-to-cell transfer is increased over a period of 8 hr. But in NRK cells, where it upregulates EGF receptors, TGFβ reduces junctional communication synergistically with EGF.

Key Words

gap junction growth factors EGF PDGF TGFβ cell-to-cell communication junctional communication 


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Copyright information

© Springer-Verlag New York Inc 1988

Authors and Affiliations

  • Pedro E. Maldonado
    • 1
  • Birgit Rose
    • 1
  • Werner R. Loewenstein
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Miami School of MedicineMiami

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