Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Identification and partial characterization of five major membrane glycoproteins of BHK fibroblasts


Five major membrane glycoproteins of the BHK-B4 hamster fibroblast plasma membrane have been identified by binding specific rabbit antibodies to the cell surface and by recovering the detergent solubilized immunocomplexes with Protein A-Sepharose immunoadsorption. These glycoproteins, designated as gp45, gp65, gp95, gp130 and gp140, are exposed at the cell surface since: (i) they were accessible to antibodies in intact viable cells; (ii) they were radioiodinated by the lactoperoxidase-glucose oxidase procedure; and (iii) they were cleaved by proteolytic enzymes in conditions affecting only the cell surface. Among these glycoproteins the gp130 is the predominant component and its exposed portion is characterized by lack of sensitivity to trypsin cleavage. Glycoproteins of different molecular weight, but immunologically related to the major hamster membrane glycoproteins, have been detected at the surface of both rat and mouse fibroblasts.

This is a preview of subscription content, log in to check access.


  1. 1.

    Bishop, J.M., Weiss, S.R., Oppermann, H., Hackett, P., Quintrell, N., Chen, L.S., Lewingtow, L., Varmus, H.E. 1978. The strategy of retroviruses gene expression.In: Avian RNA Tumor Viruses. S. Barlati and C. DeGiuli, editors p. 181. Piccin Medical Books.

  2. 2.

    Bonner, W.M., Laskey, R.A. 1974. Film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels.Eur. J. Biochem. 46:83

  3. 3.

    Carter, W.G., Hakomori, S. 1977. Isolation and partial characterization of a “Galactoproteina” (LETS) and “Galactoproteinb” from hamster embryo fibroblasts.Biochem. Biophys. Res. Commun. 76:299

  4. 4.

    Comoglio, P.M., Tarone, G., Bertini, M. 1978. Immunochemical purification of probe labelled plasma membrane proteins: An approach to the molecular anatomy of the cell surface.J. Supramol. Struc. 8:39

  5. 5.

    Diamond, L. 1967. Two spontaneously transformed cell lines derived from the same hamster embryo culture.Int. J. Cancer 2:143

  6. 6.

    Fukuda, M., Eshdad, Y., Tarone, G., Marchesi, V.T. 1978. Isolation and characterization of peptides derived from the cytoplasmic fragment of Band 3, the predominant intrinsic membrane protein of the human erythrocyte.J. Biol. Chem. 253:2419

  7. 7.

    Gupta, S.L., Goldstein, G., Boyse, E.A. 1977. Accessibility of plasma membrane antigens.Immunogenetics 5:379

  8. 8.

    Hedman, K., Vaheri, A., Watiovaara, J. 1978. External fibronectin of cultured human fibroblasts is predominantly a matrix protein.J. Cell Biol. 76:748

  9. 9.

    Hughes, R.C., Narin, R. 1978. Labelling, solubilization and peptide mapping of fibroblast surface glycoprotein.Ann. N. Y. Acad. Sci. 312:192

  10. 10.

    Hynes, R.O. 1973. Alteration of cell surface proteins by viral transformation and by proteolysis.Proc. Nat. Acad. Sci. 70:3170

  11. 11.

    Hynes, R.O. 1976. Cell surface proteins and malignant transformation.Biochim. Biophys. Acta 458:73

  12. 12.

    Isaka, T., Ikawa, Y., Yoshida, M. 1978. Characterization of transformation sensitive membrane proteins in chick embryo fibroblasts transformed by avian sarcoma viruses.Virology 90:330

  13. 13.

    Kessler, S.W. 1976. Cell membrane antigen isolation with the staphylococcal Protein-A adsorbent.Immunology.177:1482

  14. 14.

    Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T.Nature (London) 227:680

  15. 15.

    Laskey, R.A., Mills, A.D. 1975. Quantitative film detection of3H and14C in polyacrylamide gels by fluorography.Eur. J. Biochem. 56:335

  16. 16.

    Marchesi, V.T., Furthmayr, H., Tomita, M. 1976. The red cell membrane.Annu. Rev. Biochem. 45:667

  17. 17.

    Murphy, H. 1977. A new replication defective variant of the Bryan high titre strain of Rous sarcoma virus.Virology 77:705

  18. 18.

    Pena, S.D., Hughes, R.C. 1978. Fibronectin-plasma membrane interactions in the adhesion and spreading of hamster fibroblasts.Nature (London) 276:80

  19. 19.

    Pena, S.D., Mills, G., Hughes, R.C. 1979. Two-dimensional electrophoresis of surface glycoproteins of normal BHK cells and Ricin resistant mutants.Biochim. Biophys. Acta 55:100

  20. 20.

    Pouyssegur, J., Shiu, R.P., Pastan, I. 1977. Induction of two transformation sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation.Cell 11:941

  21. 21.

    Pouyssegur, J., Yamada, K.M. 1978. Isolation and immunological characterization of a glucose-regulated fibroblasts cell surface glycoprotein and its non glycosylated precursor.Cell 13:139

  22. 22.

    Ruoslahti, E., Vaheri, A. 1974. A novel human serum protein from fibroblast plasma membrane.Nature (London) 248:790

  23. 23.

    Shiu, R.P., Pouyssegur, J., Pastan, I. 1977. Glucose depletion account for the induction of two transformation sensitive membrane proteins in Rous sarcoma virus transformed chick embryo fibroblasts.Proc. Nat. Acad. Sci. 74:3840

  24. 24.

    Steck, T.L., Ramos, B., Strapazon, E. 1976. Proteolytic dissection of Band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane.Biochemistry 15:1154

  25. 25.

    Stoker, M., Macpherson, I. 1964. Syrian hamster fibroblast cell line BHK-21 and its derivative.Nature (London) 203:1355

  26. 26.

    Stone, K.R., Smith, R.E., Joklik, W.K. 1974. Changes in membrane polypeptides that occur when chick embryo fibroblasts and NRK cells are transformed with avian sarcoma viruses.Virology 58:86

  27. 27.

    Svoboda, J. 1964. Malignant interactions of Rous sarcoma virus with mammalian cells in vivo and in vitro.Nat. Cancer Inst. Monogr. 17:277

  28. 28.

    Tarone, G., Comoglio, P.M. 1977. Plasma membrane proteins exposed at the outer surface of control and Rous sarcoma virus transformed hamster fibroblasts.Exp. Cell Res. 110:143

  29. 29.

    Warren, L., Fuhrer, J.P., Buck, C.A. 1972. Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth dependent sialyl-transferase.Proc. Nat. Acad. Sci. 69:1838

  30. 30.

    Yamada, K.M., Olden, K. 1978. Fibronectin-adhesive glycoproproteins of the cell surface and blood.Nature (London) 275:179

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tarone, G., Prat, M. & Comoglio, P.M. Identification and partial characterization of five major membrane glycoproteins of BHK fibroblasts. J. Membrain Biol. 53, 55–61 (1980).

Download citation


  • Molecular Weight
  • Plasma Membrane
  • Viable Cell
  • Cell Surface
  • Trypsin