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Pharmacy World and Science

, Volume 15, Issue 6, pp 263–268 | Cite as

Mucuna pruriens: Improvement of the biotechnological production of the anti-Parkinson drug L-dopa by plant cell selection

  • Niesko Pras
  • Herman J. Woerdenbag
  • Sieb Batterman
  • Jan F. Visser
  • Wim Van Uden
Articles

Abstract

Routinely grown cell suspension cultures ofMucuna pruriens L. (Fabaceae) were able to endogenously accumulate the anti-Parkinson drug L-dihydroxyphenylalanine (L-dopa) in the range between 0.2 and 2% on a dry weight (DW) basis. The green colour that developed in light-exposed cultures, appeared to be a suitable marker to select cells with an increased L-dopa biosynthesis and/or phenoloxidase activity. For this purpose, saccharose concentrations from 0 to 4% (w/v), and light intensities of 1,000 and 2,000 lux, were involved in the selection procedure. After 6 months, photomixotrophic callus cultures with a rapid growth and a high L-dopa content of 0.9% (DW) were obtained on 2% saccharose and under 1,000 lux. The cell suspensions, derived from these calli, accumulated up to 6% (DW) L-dopa, which was the highest stable content ever measured in cultures ofM. pruriens. An L-dopa yield of approximately 1.2 g/l was calculated after 6 days of growth. In contrast, compared with the standard-grown parent cell line, the phenoloxidase activity, and consequently the bioconversion capacity as measured after entrapment in calcium alginate, of these high-producing cultures was approximately threefold lower.

Keywords

Cells, cultured Levodopa Mucuna pruriens Selection (genetics) 

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Copyright information

© Royal Dutch Association for the Advancement of Pharmacy 1993

Authors and Affiliations

  • Niesko Pras
    • 1
  • Herman J. Woerdenbag
    • 1
  • Sieb Batterman
    • 1
  • Jan F. Visser
    • 1
  • Wim Van Uden
    • 1
  1. 1.Department of PharmacognosyUniversity Centre for Pharmacy, Groningen UniversityAW GroningenThe Netherlands

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