The Journal of Membrane Biology

, Volume 89, Issue 1, pp 65–73 | Cite as

Demarcation of Ca2+ transport processes in guinea pig stomach smooth muscle

  • Yasushi Sakai
  • Akiyo Isobe
  • Santa Ichikawa
Articles
Received:
Revised:

Summary

Microsomal fractions were isolated from gastric antrum and fundus smooth muscle of guinea pigs. Ca2+ uptake into and Ca2+ release from the membrane vesicles were studied by a rapid filtration method, and Ca2+ transport properties of the different regions of the stomach were compared. ATP-dependent Ca2+ uptake was similar in microsomes isolated from both regions. This uptake was increased by oxalate and was not affected by NaN3. Oxalate affected Ca2+ permeability of both antrum and fundus microsome vesicles similarly. Fundus microsome vesicles preincubated in 100mm NaCl and then diluted to 1/20 concentration with Na+-free medium had significantly higher ATP-independent Ca2+ uptake than vesicles preincubated in 100mm KCl and treated the same way. This was not true for antrum vesicles. Monensin abolished Na+-dependent Ca2+ uptake, and NaCl enhanced Ca2+ efflux from fundus microsome vesicles. The halflife values of Ca2+ loss from fundus vesicles in the presence of NaCl were significantly smaller than those in the presence of KCl. The release of Ca2+ from the vesicles within the first 3 min was accelerated by NaCl to three times that by KCl. However, NaCl had ro effect on Ca2+ release from antrum microsome vesicles.

Results suggest two distinct mechanisms of stomach membrane Ca2+ transport: (1) ATP-dependent Ca2+ uptake and (2) Na+−Ca2+ exchange; the latter in the fundus only.

Key Words

concentration-relaxation Ca2+ transport Na+−Ca2+ exchange microsome smooth muscle 
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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Yasushi Sakai
    • 1
  • Akiyo Isobe
    • 1
  • Santa Ichikawa
    • 1
  1. 1.2nd Department of PhysiologyShowa University School of MedicineTokyoJapan

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