The Journal of Membrane Biology

, Volume 59, Issue 1, pp 57–63 | Cite as

Effects of quinine on Ca++-induced K+ efflux from human red blood cells

  • Esther Reichstein
  • Aser Rothstein
Articles

Summary

The Ca++-mediated increase in K+-permeability of intact red blood cells (Gardos effect) was initiated by exposing cells to known concentrations of Ca++ (using EGTA buffers) in the presence of the ionophore A23187. The potency of quinine, an inhibitor of the response, was found to depend on the external K+ concentration. In K+-free solutions the concentration of quinine to achieve 50% inhibition (K50) was 5 μm, but at 5mm K+ the required concentration was increased 20-fold to 100 μm. An increase in internal Na+ had the opposite effect, allowing a high potency of quinine despite the presence of external K+. Alterations in the internal K+ level, on the other hand, were without effect on theK50, suggesting that the membrane potential is not a factor. This conclusion is supported by the lack of effect on quinine inhibition of substitution of Cl by NO3, a considerably more permeant anion. The data are consistent with the hypothesis that quinine inhibits by competitively displacing K+ from an external binding site, the reported K+-activation site for the Ca++-mediated K+-permeability.

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Copyright information

© Springer-Verlag New York Inc 1981

Authors and Affiliations

  • Esther Reichstein
    • 1
  • Aser Rothstein
    • 1
  1. 1.Research InstituteThe Hospital for Sick ChildrenTorontoCanada

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