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Microenvironment changes of human blood platelet membranes associated with fibrinogen binding

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Summary

Alterations in the membrane organization caused by fibrinogen binding to human blood platelets and their isolated membranes were analyzed by fluorescence and electron spin resonance measurements. The degree of fluorescent anisotropy of DPH, ANS and fluorescamine increased significantly when fibrinogen reacted with its membrane receptors. Both fluorescence and ESR analyses showed that fibrinogen binding to platelet membranes is accompanied by an increase of the membrane lipid rigidity. This effect seems to be indirect in nature and is mediated by altered membrane protein interactions. As it has been shown that an increased membrane lipid rigidity leads to a greater exposure of membrane proteins, including fibrinogen receptors, this might facilitate a formation of molecular linkages between neighboring platelets. On the other hand, changes of fluorescence anisotropy of membrane tryptophans and N-(3-pyrene) maleimide suggest the augmented mobility of the membrane proteins. Evidence is presented which indicated that the binding of fibrinogen to the membrane receptors is not accompanied by any changes in the fluorescence intensity of ANS attached to the membranes. It may suggest that the covering of platelets with fibrinogen does not influence the surface membrane charge. In contrast to fibrinogen, calcium ions caused an increase of the fluorescence intensity resulting from the more efficient binding of ANS to the platelet membranes.

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References

  1. Andley, U.P., Chakrabarti, B. 1981. Interaction of 8-anilino-1-naphthalenesulfonate with rod outer segment membrane.Biochemistry 20:1687–1693

  2. Barber, A.J., Jamieson, G.A. 1970. Isolation and characterization of plasma membranes from human blood platelets.J. Biol. Chem. 245:6357–6365

  3. Boggs, J.M., Vail, W.J., Moscarello, M.A. 1976. Preparation and properties of vesicles of a purified myelin hydrophobic protein and phospholipid. A spin label study.Biochim. Biophys. Acta 448:517–530

  4. Bouchy, M., Donner, M., André, J.C. 1981. Evolution of fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) during the labelling of living cells.Exp. Cell Res. 133:39–46

  5. Chen, R.F., Bowman, R.L. 1965. Fluorescence polarization measurement with ultraviolet-polarizing filters in a spectrophotofluorometer.Science 147:729–732

  6. Cierniewski, C.S., Kowalska, M.A., Krajewski, T., Janiak, A. 1982. Binding of fibrinogen molecules to pig platelets and their membranes.Biochim. Biophys. Acta 714:543–548

  7. Cierniewski, C.S., Krajewski, T., Wodzinowska, B. 1976. Glycoproteins of mammalian platelet membranes.Thromb. Haemost. 35:264–267

  8. Cogan, U., Shinitzky, M., Weber, G., Nishida, T. 1973. Microviscosity and order in the hydrocarbon region of phospholipid and phospholipid-cholesterol dispersion determined with fluorescent probes.Biochemistry 12:521–528

  9. Doolittle, R.F., Schubert, D., Schwartz, S.A. 1967. Amino acid sequence studies on artiodactyl fibrinopeptides. I. Dromedary camel, mule deer, and cape buffalo.Arch. Biochem. Biophys. 118:456–467

  10. Galla, H.J., Hartman, W. 1980. Excimer-forming lipids in membrane research.Chem. Phys. Lipids 27:199–219

  11. Galla, H.J., Luisetti, J. 1980. Lateral and transversal diffusion and phase transitions in erythrocyte membranes. An excimer fluorescence study.Biochim. Biophys. Acta 596:108–117

  12. Galla, H.J., Sackman, E. 1974. Lateral diffusion in the hydrophobic region of membranes. Use of pyrene excimers as optical probes.Biochim. Biophys. Acta 339:103–115

  13. Gordon, L.M., Sauerheber, R.D. 1977. Studies on spin-labelled egg lecithin dispersions.Biochim. Biophys. Acta 466:34–43

  14. Hashimoto, S., Shibata, S., Kobayashi, B. 1977. Exterior and interior events in early stage of thrombin-induced platelet aggregation.Thromb. Haemost. 38:630–639

  15. Hawkes, S.P., Meehan, T.D., Bissell, J.J. 1976. The use of fluorescamine as a probe for labelling the outer surface of the plasma membrane.Biochem. Biophys. Res. Commun. 68:1226–1233

  16. Hubbell, W.L., McConnell, H.M. 1971. Molecular motion in spin-labelled phospholipids and membranes.J. Am. Chem. Soc. 93:314–326

  17. Inbar, M., Shinitzky, M. 1975. Decrease in microviscosity of lymphocyte surface membrane associated with stimulation induced by concanavalin A.Eur. J. Immunol. 5:166–170

  18. Liu, B.M., Cheung, H.C., Chem, K.H., Habercom, M.S. 1980. Fluorescence decay kinetics of pyrene in membrane vesicles.Biophys. Chem. 12:341–355

  19. Marguerie, G.A., Edgington, T.S., Plow, E.F. 1980. Interaction of fibrinogen with its platelet receptor as part of a multistep reaction in ADP-induced platelet aggregation.J. Biol. Chem. 255:154–161

  20. Marguerie, G.A., Plow, E.F., Edgington, T.S. 1979. Human platelets possess an inducible and saturable receptor specific for fibrinogen.J. Biol. Chem. 254:5357–5363

  21. Mihalyi, E. 1968. Physicochemical studies of bovine fibrinogen. IV. Ultraviolet absorption and its relation to the structure of the molecule.Biochemistry 7:208–223

  22. Mustard, J.F., Petry, D.W., Ardlie, N.C., Packham, M.A. 1977. Preparation of suspensions of washed platelets from humans.Br. J. Haematol. 22:193–204

  23. Nachman, R.L., Ferris, B. 1974. Binding of adenosine diphosphate by isolated membranes from human platelets.J. Biol. Chem. 249:704–710

  24. Nathan, I., Fleischer, G., Dvilansky, A., Livne, A., Parola, A.H. 1980. Membrane dynamic alterations associated with activation of human platelets by thrombin.Biochim. Biophys. Acta 598:417–421

  25. Nathan, I., Fleisher, G., Livne, A., Dvilansky, A., Parola, A.H. 1979. Membrane microenvironmental changes during activation of human blood platelets by thrombin.J. Biol. Chem. 254:9822–9828

  26. Niewiarowski, S., Budzynski, A.Z., Morinelli, T.A., Steward, G.J., Brudzyński, T.M. 1981. Exposure of fibrinogen receptor on human platelets by proteolitic enzymes.J. Biol. Chem. 256:917–925

  27. Radda, G.K. 1975. Fluorescence probes in membrane studies.In: Methods in Membrane Biology. E.D. Korn, editor. pp. 97–188. Plenum, New York

  28. Radda, G.K., Vanderkooi, J. 1972. Can fluorescent probes tell us anything about membranes?Biochim. Biophys. Acta 265:509–549

  29. Sauerheber, R.D., Zimmerman, T.S., Esgate, J.A., Vanderlaan, W.P., Gordon, L.M. 1980. Effects of calcium, lathanum and temperature on the fluidity of spin-labeled human platelets.J. Membrane Biol. 52:201–219

  30. Seelig, J. 1970. Spin label studies of oriented smectic liquid crystals. A model system for bilayer membranes.J. Am. Chem. Soc. 92:3881–3887

  31. Shinitzky, M., Inbar, M. 1976. Microviscosity parameters and protein mobility in biological membranes.Biochim. Biophys. Acta 433:133–149

  32. Steiner, M. 1981. Vitamin E changes the membrane fluidity of human platelets.Biochim. Biophys. Acta 640:100–105

  33. Tangen, O., Berman, H.J., Marfey, P. 1971. Gel filtration: A new technique for separation of blood platelets from plasma.Thromb. Diath. Haemorrh. 25:268–278

  34. Udenfriend, S., Stein, S., Böhlen, P., Dairman, W., Teimbruber, W., Weigele, M. 1972. Fluorescamone: A reagent for assay of amino acids, peptides, proteins and primary amines in the picomole range.Science 178:871–872

  35. Vanderkooi, J.M., Fichkoff, S., Andrich, M., Podo, F., Owen, C.S. 1975. Diffusion in two dimensions: Comparison between diffusional fluorescence quenching in phospholipid vesicles and in isotropic solution.J. Chem. Phys. 63:3361–3366

  36. Weltman, J.K., Sharo, R.P. 1973. N-(3-pyrene)maleimide: A long lifetime fluorescent sufhydryl reagent.J. Biol. Chem. 248:3173–3177

  37. Wu, Ch-W., Yarbough, L.R., Wu, F.Y.-H. 1976. N-(1-pyrene) maleimide: A fluorescent cross-linking reagent.Biochemistry 15:2863–2868

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Kowalska, M.A., Cierniewski, C.S. Microenvironment changes of human blood platelet membranes associated with fibrinogen binding. J. Membrain Biol. 75, 57–64 (1983). https://doi.org/10.1007/BF01870799

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Key Words

  • fibrinogen
  • platelet membranes
  • spectroscopy
  • fluorescent probes