The Journal of Membrane Biology

, Volume 64, Issue 1–2, pp 91–102 | Cite as

Aldosterone control of the density of sodium channels in the toad urinary bladder

  • Lawrence G. Palmer
  • Jack H. Y. Li
  • Bernd Lindemann
  • Isidore S. Edelman


Near-instantaneous current-voltage relationships and shot-noise analysis of amiloride-induced current fluctuations were used to estimate apical membrane permeability to Na (PNa), intraepithelial Na activity (Na c ), single-channel Na currents (i) and the number of open (conducting) apical Na channels (N0), in the urinary bladder of the toad (Bufo marinus). To facilitate voltageclamping of the apical membrane, the serosal plasma membranes were depolarized by substitution of a high KCl (85mm) sucrose (50mm) medium for the conventional Na-Ringer's solution on the serosal side.

Aldosterone (5×10−7m, serosal side only) elicited proportionate increases in the Na-specific current (INa and inPNa, with no significant change in the dependence ofPNa on mucosal Na (Na o ).PNa and the control ofPNa by aldosterone were substrate-dependent: In substrate-depleted bladders, pretreatment with aldosterone markedly augmented the response to pyruvate (7.5×10−3m) which evoked coordinate and equivalent increases inINa andPNa.

The aldosterone-dependent increase inPNa was a result of an equivalent increase in the area density of conducting apical Na channels. The computed single-channel current did not change. We propose that, following aldosterone-induced protein synthesis, there is a reversible metabolically-dependent recruitment of preexisting Na channels from a reservoir of electrically undetectable channels. The results do not exclude the possibility of a complementary induction of Na-channel synthesis.

Key words

transepithelial Na transport apical Na permeability Na-channel density aldosterone 


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Copyright information

© Springer-Verlag New York Inc. 1982

Authors and Affiliations

  • Lawrence G. Palmer
    • 1
    • 2
  • Jack H. Y. Li
    • 1
    • 2
  • Bernd Lindemann
    • 1
    • 2
  • Isidore S. Edelman
    • 1
    • 2
  1. 1.Department of Biochemistry, College of Physicians and SurgeonsColumbia UniversityNew York
  2. 2.II. Physiologisches Institut der Universität des SaarlandesHomburgWest Germany

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