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The Journal of Membrane Biology

, Volume 64, Issue 1–2, pp 77–89 | Cite as

The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone

  • Jack H. Y. Li
  • Lawrence G. Palmer
  • Isidore S. Edelman
  • Bernd Lindemann
Articles

Summary

Urinary bladders ofBufo marinus were depolarized, by raising the serosal K concentration, to facilitate voltage-clamping of the apical membrane. Passive Na transport across the apical membrane was then studied with near-instantaneous current-voltage curves obtained before and after eliciting a natriferic response with oxytocin. Fitting with the constant-field equation showed that the natriferic effect is accounted for by an increase in the apical Na permeability. It is accompanied by a small increase in cellular Na activity. Furthermore, fluctuation analysis of the amiloride-induced shot-noise component of the short-circuit current indicated that the permeability increase is not due to increased Na translocation through those Na channels which were already conducting prior to hormonal stimulation. Rather, the natriferic effects is found to be based on an increase in the population of transporting channels. It appears that, in response to the hormone, Na channels are rapidly “recruited” from a pool of electrically silent channels.

Key words

transepithelial Na transport apical Na perme-ability Na-channel density oxytocin 

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Copyright information

© Springer-Verlag New York Inc. 1982

Authors and Affiliations

  • Jack H. Y. Li
    • 1
    • 2
  • Lawrence G. Palmer
    • 1
    • 2
  • Isidore S. Edelman
    • 1
    • 2
  • Bernd Lindemann
    • 1
    • 2
  1. 1.II. Physiologisches Institut der Universität des SaarlandesHomburg/SaarWest Germany
  2. 2.Department of Biochemistry, College of Physicians and SurgeonsColumbia UniversityNew York

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