The Journal of Membrane Biology

, Volume 64, Issue 1–2, pp 77–89 | Cite as

The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone

  • Jack H. Y. Li
  • Lawrence G. Palmer
  • Isidore S. Edelman
  • Bernd Lindemann


Urinary bladders ofBufo marinus were depolarized, by raising the serosal K concentration, to facilitate voltage-clamping of the apical membrane. Passive Na transport across the apical membrane was then studied with near-instantaneous current-voltage curves obtained before and after eliciting a natriferic response with oxytocin. Fitting with the constant-field equation showed that the natriferic effect is accounted for by an increase in the apical Na permeability. It is accompanied by a small increase in cellular Na activity. Furthermore, fluctuation analysis of the amiloride-induced shot-noise component of the short-circuit current indicated that the permeability increase is not due to increased Na translocation through those Na channels which were already conducting prior to hormonal stimulation. Rather, the natriferic effects is found to be based on an increase in the population of transporting channels. It appears that, in response to the hormone, Na channels are rapidly “recruited” from a pool of electrically silent channels.

Key words

transepithelial Na transport apical Na perme-ability Na-channel density oxytocin 


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  1. Aceves, J., Cuthbert, A.W., Edwardson, J.M. 1979. Estimation of the density of sodium entry sites in frog skin epithelium from the uptake of [3H]-benzamil.J. Physiol. (London) 295:477–490Google Scholar
  2. Andersen, B., Ussing, H.H. 1957. Solvent drag on nonelectrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone.Acta Physiol. Scand. 39:228–239PubMedGoogle Scholar
  3. Andreoli, T.E., Schafer, J.A. 1976. Mass transport across cell membranes: The effects of antidiuretic hormone on water and solute flows in epithelia.Annu. Rev. Physiol. 38:451–500PubMedGoogle Scholar
  4. Begenisich, T., Stevens, C.F. 1975. How many conductance states do potassium channels have?Biophys. J. 15:843–846PubMedGoogle Scholar
  5. Biber, T.U.L., Cruz, L.J. 1973. Effect of antidiuretic hormone on sodium uptake across outer surface of frog skin.Am. J. Physiol. 225:912–917Google Scholar
  6. Cereijido, M., Herrera, F.C., Flanigan, W.J., Curran, P.F. 1964. The influence of Na-concentration on Na-transport across frog skin.J. Gen. Physiol. 47:879–893PubMedGoogle Scholar
  7. Curran, P.F., Herrera, F.C., Flanigan, W.J. 1963. The effect of Ca and antidiuretic hormone on Na-transport across frog skin. II. Sites and mechanisms of action.J. Gen. Physiol. 46:1011–1027Google Scholar
  8. Cuthbert, A.W. 1976. Amiloride as a probe for sodium entry sites in frog skin epithelium.J. Physiol. (London) 266:28PGoogle Scholar
  9. Cuthbert, A.W., Painter, E. 1969. Capacitance changes in frog skin caused by theophylline and antidiuretic hormone.Brit. J. Pharmacol. 37:314–324Google Scholar
  10. Cuthbert, A.W., Shum, W.K. 1974. Amiloride and the sodium channel.Naunyn Schmiederbergs Arch. Pharmacol. 281:261–269Google Scholar
  11. Cuthbert, A.W., Shum, W.K. 1975. Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells.Proc. R. Soc. London B 189:543–575Google Scholar
  12. DiBona, D.R. 1978. Direct visualization of epithelial morphology in the living amphibian urinary bladder.J. Membrane Biol. Special Issue:45–70Google Scholar
  13. Eigler, J., Kelter, J., Renner, E. 1967. Wirkungscharakteristika eines neuen Acylquanidins-Amilorid-HCl (MK 870) an der isolierten Haut von Amphibien.Klin. Wochenschr. 45:737–738PubMedGoogle Scholar
  14. Ekblad, E.B.M., Strum, J.M., Edelman, I.S. 1976. Differential covalent labeling of apical and basal-lateral membranes of the epithelium of the toad bladder.J. Membrane Biol. 26:301–317Google Scholar
  15. Frazier, H.S., Dempsey, E.F., Leaf, A. 1962. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.J. Gen. Physiol. 45:529–543Google Scholar
  16. Fuchs, W., Hviid Larsen, E., Lindemann, B. 1977. Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.J. Physiol. (London) 267:137–166Google Scholar
  17. Furhmann, F.A., Ussing, H.H. 1951. A characteristic response of the isolated frog skin potential to neurohypophyseal principles and its relation to the transport of sodium and water.J. Cell. Comp. Physiol. 38:109–130Google Scholar
  18. Gronowicz, G., Masur, S.K., Holtzman, E. 1980. Quantitative analysis of exocytosis and endocytosis in the hydroosmotic response of toad bladder.J. Membrane Biol. 52:221–235Google Scholar
  19. Jörgensen, C.B., Levi, H., Ussing, H.H. 1946. On the influence of neurohypophyseal principles on the sodium metabolism in the axolotl (Amblystoma mexicanuum).Acta Physiol. Scand. 12:350–371Google Scholar
  20. Kachadorian, W.A., Levine, S.D., Wade, J.B., DiScala, V.A., Hays, R.M. 1977. Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.J. Clin. Invest. 59:576–581PubMedGoogle Scholar
  21. Li, J.H.-Y., Palmer, L.G., Edelman, I.S., Lindemann, B. 1979. Effect of ADH on Na-channel parameters in toad urinary bladder.Pfluegers Arch. 382:R13Google Scholar
  22. Lindemann, B., DeFelice, L.J. 1981. On the use of general network functions in the evaluation of noise spectra obtained from epithelia.In: Ion Transport by Epithelia: Recent Advances. S.G. Schultz, editor. Raven Press, New York (in press)Google Scholar
  23. Lindemann, B., Van Driessche, W. 1977. Sodium specific membrane channels of frog skin are pores: Current fluctuations reveal high turnover.Science 195:292–294PubMedGoogle Scholar
  24. Lindemann, B., Van Driessche, W. 1978. The mechanism of Na-uptake through Na-selective channels in the epithelium of frog skin.In: Membrane Transport Processes. J.F. Hoffman, editor. Vol. 1, p. 155. Raven Press, New YorkGoogle Scholar
  25. Macknight, A.D.C., DiBona, D.R., Leaf, A. 1980. Sodium transport across toad urinary bladder: A model “tight” epithelium.Physiol. Rev. 60:615–715Google Scholar
  26. Mandel, L.J. 1978. Effects of pH, Ca, ADH, and theophylline on kinetics of Na-entry in frog skin.Am. J. Physiol. 235:C35-C48Google Scholar
  27. Muller, J., Kachadorian, W.A., DiScala, V.A. 1980. Evidence that ADH-stimulated intramembrane particle aggregates are transferred from cytoplasmic to luminal membranes in toad bladder epithelial cells.J. Cell Biol. 85:83–95PubMedGoogle Scholar
  28. Orloff, J., Handler, J. 1962. The similarity of effects of vasopressin, adenosine-3′,5′-phosphate (cyclic AMP) and theophylline on the toad bladder.J. Clin. Invest. 41:702–709PubMedGoogle Scholar
  29. Orloff, J., Handler, J. 1967. The role of adenosine 3′,5′-phosphate in the action of antidiuretic hormone.Am. J. Med. 42:757–768PubMedGoogle Scholar
  30. Palmer, L.G., Edelman, I.S. 1981. Control of apical Na permeability in the toad urinary bladder by aldosterone.Ann. N.Y. Acad. Sci. (in press) Google Scholar
  31. Palmer, L.G., Edelman, I.S., Lindemann, B. 1980. Current-voltage analysis of apical sodium-transport in toad urinary bladders: Effects of inhibitors of transport and metabolism.J. Membrane Biol. 57:59–71Google Scholar
  32. Palmer, L.G., Li, J.H.-Y., Lindemann, G., Edelman, I.S. 1982. Aldosterone control of the density of sodium channels in the toad urinary bladder.J. Membrane Biol. 64:91–102Google Scholar
  33. Roloff, C., Dörge, A., Rick, R., Thurau, K. 1978. Effect of vasopressin on intracellular electrolyte composition of the frog skin.Pfluegers Arch. 377:R40Google Scholar
  34. Stetson, D.L., Lewis, S.A., Wade, J.B. 1981. ADH-induced increase in transepithelial capacitance in toad bladder.Biophys. J. 33:43aGoogle Scholar
  35. Van Driessche, W., Hegel, U. 1978. Amiloride induced fluctuations of short circuit current through toad urinary bladder. 6th International Biophysics Congress. Kyoto, Japan. p. 215Google Scholar
  36. Van Driessche, W., Lindemann, B. 1978. Low noise amplification of voltage and current fluctuations arising in epithelia.Rev. Sci. Instrum. 49:52–55Google Scholar
  37. Van Driessche, W., Lindemann, B. 1979. Concentration dependence of currents through single sodium-selective pores in frog skin.Nature (London) 282:519–520Google Scholar
  38. Wade, J.B. 1978. Membrane structural specialization of the toad urinary bladder revealed by the freeze-fracture technique. III. Location, structure and vasopressin dependence of intramembrane particle arrays.J. Membrane Biol. Special Issue: 281–296Google Scholar
  39. Warnche, J., Lindemann, B. 1979. A sinewave-burst method to obtain impedance spectra of transporting epithelia during voltage clamp.Pfluegers Arch. 382:R12Google Scholar
  40. Warncke, J., Lindemann, B. 1980. Effect of ADH on the capacitance of apical epithelial membranes.Proc. 28th Int. Congr. Physiol. Sci. (Budapest) pp. 129–133.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1982

Authors and Affiliations

  • Jack H. Y. Li
    • 1
    • 2
  • Lawrence G. Palmer
    • 1
    • 2
  • Isidore S. Edelman
    • 1
    • 2
  • Bernd Lindemann
    • 1
    • 2
  1. 1.II. Physiologisches Institut der Universität des SaarlandesHomburg/SaarWest Germany
  2. 2.Department of Biochemistry, College of Physicians and SurgeonsColumbia UniversityNew York

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