The Journal of Membrane Biology

, Volume 64, Issue 1–2, pp 11–21 | Cite as

Membrane transport during erythroid differentiation

  • Portia B. Gordon
  • Meryl S. Rubin
Articles
Received:
Revised:

Summary

Transport, unidirectional flux, of a monosaccharide, a nucleoside and three amino acids, all of which enter cells by independent, discrete carriers, was compared at three stages of erythroid maturation, the normal (anucleate) mouse erythrocyte, and in differentiated and undifferentiated Friend erythroleukemia cells. We found specific transport alterations during this developmental program. Transport of 3-O-methylglucose increased with each successive developmental stage. Aminoisobutyrate transport was maintained during Friend cell differentiation, but fell slightly in erythrocytes. Leucine, lysine and uridine transport began to fall two days after dimethylsulfoxide exposure, and diminished further in red cells. These studies of transport are not directly comparable to uptake studies reported by others.

Median cell volume and thus surface area decreased more during differentiation than amino acid transport declined, so flux, transport past a unit area of membrane, actually increased. Monosaccharide flux also increased. Only uridine transport fell in parallel to surface area. Perhaps sites for nutrient transport required for energy production are preferentially maintained.

Key words

Friend cells membrane transport erythroid differentiation 
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Copyright information

© Springer-Verlag New York Inc. 1982

Authors and Affiliations

  • Portia B. Gordon
    • 1
  • Meryl S. Rubin
    • 1
  1. 1.Departments of Molecular Pharmacology, Medicine and BiochemistryAlbert Einstein College of MedicineBronx

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