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The Journal of Membrane Biology

, Volume 74, Issue 1, pp 41–49 | Cite as

Single channel K+ currents from HeLa cells

  • R. Sauvé
  • G. Roy
  • D. Payet
Articles

Summary

The extracellular patch-clamp technique was used in order to investigate the presence of ionic channels in HeLa cells, a well-known cultured cell type obtained from an epidermoid carcinoma of the cervix. Under Gigohm-seal conditions, discrete current jumps could be observed with patch electrodes containing KCl. These channels were found to be mainly permeable to K+ and showed multiple levels of conductance. From single-channelI–V curve measurements, a strong rectification effect, characterized by a large inward and no detectable outward current, was observed. For negative membrane potentials (0 to −90 mV), the measured current-voltage relationship was found to be mostly linear, corresponding to a single-channel conductance of 40 pS. An analysis of some selected time records has revealed in addition that the probability of the channel to be in the open state was a function of the KCl concentration in the patch pipette.

Key Words

HeLa cells single channel patch clamp potassium inward reetification Ca++ activated 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • R. Sauvé
    • 1
  • G. Roy
    • 2
  • D. Payet
    • 3
  1. 1.Department of PhysiologyUniversity of MontrealMontreal
  2. 2.Department of PhysicsUniversity of MontrealMontreal
  3. 3.Department of Biophysics, Faculty of MedicineUniversity of SherbrookeSherbrookeCanada

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