The Journal of Membrane Biology

, Volume 77, Issue 3, pp 213–222 | Cite as

Current-voltage relations of the basolateral membrane in tight amphibian epithelia: Use of nystatin to depolarize the apical membrane

  • Haim Garty
Articles
Received:
Revised:

Summary

Exposure of the mucosal side of toad(Bufo bufo) urinary bladder and frog(Rana ridibunda) skin to the polyene ionophore nystatin, resulted in stable preparations in which the apical resistance was negligible compared to the basolateral resistance. The preparations support passive K currents in both directions and an amiloride-insensitive Na current in the apicalserosal direction which is blocked by ouabain. The nystatintreated toad bladder was used to study the electrical properties of the basolateral membrane by means of current-voltage curves recorded transepithelially. The K current showed strong rectification at cellular potentials negative with respect to the interstitial space. The ouabain-sensitive current increased with membrane voltage at negative voltages but saturated above+20 mV.

Key Words

basolateral membrane current-voltage relations nystatin potassium transport tight epithelia 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Haim Garty
    • 1
  1. 1.Department of Membrane ResearchThe Weizmann Institute of ScienceRehovotIsrael

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