The Journal of Membrane Biology

, Volume 73, Issue 1, pp 61–68 | Cite as

Effects of acid base disturbances on basolateral membrane potential and intracellular potassium activity in the proximal tubule ofNecturus

  • Takahiro Kubota
  • Bruce A. Biagi
  • Gerhard Giebisch
Articles
Received:
Revised:

Summary

The effects of extracellular acid-base disturbances on intracellular potential (Em) and potassium activity (a K i ) in the early proximal tubule ofNecturus were examined. Using conventional and double barreled potassium ion selective microelectrodes it was possible to measure both the transient and steady-state responses to various states of extracellular acidosis and alkalosis. The results show that (i) when extracellular [HCO 3 − ] is varied at constantpCO2,Em anda K i decrease in acidosis and increase in alkalosis. The greatest sensitivity inEm is between pH 7.6 and 6.8 with apparent saturation above and below these extremes; (ii) decreased [HCO 3 − ] at constant pH=7.6 also causes a depolarization ofEm and reducesa K i , suggesting a major effect of extracellular [HCO 3 − ] on intracellular potential anda K i ; (iii) rapid perfusions and transient ΔEm analysis suggest a high basolateral conductance for K+ and HCO 3 − and a low Cl− conductance; (iv) increasing extracellular [K+] decreases the response of bothEm anda K i to reduced [HCO 3 − ] at constantpCO2. The results of this study demonstrate the important role of extracellular pH and/or [HCO 3 − ] on the maintenance of cellular K+ homeostasis.

Key Words

acidosis alkalosis pH bicarbonate 
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References

  1. Adler, S., Farley, D.S. 1977. Potassium and intracellular pH.Kidney Int. 11:433–442Google Scholar
  2. Anagnostopoulos, T. 1972. Effects of pH on electrical properties of peritubular membrane and shunt pathway ofNecturus kidney.In: Abstracts of the 5th International Congress of Nephrology. (Mexico City) p. 104Google Scholar
  3. Biagi, B., Kubota, T., Sohtell, M., Giebisch, G. 1981a. Intracellular potentials in rabbit proximal tubules perfusedin vitro.Am. J. Physiol. 240:F200-F210Google Scholar
  4. Biagi, B., Sohtell, M., Giebisch, G. 1981b. Intracellular potassium activity in the rabbit proximal straight tubule.Am. J. Physiol. 241:F677-F686Google Scholar
  5. Boron, W.F., Boulpaep, E.L. 1980. Intracellular pH in isolated, perfused proximal tubules of amphibian kidney.Fed. Proc. 39:713 (Abstr.)Google Scholar
  6. Boron, W.F., Boulpaep, E.L. 1981. Basolateral bicarbonate transport in isolated, perfused renal proximal tubules of the salamander.Fed. Proc. 40:705Google Scholar
  7. Boulpaep, E.L., Giebisch, G. 1978. Electrophysiological measurements on the renal tubule.In: Methods in Pharmacology. Manuel Martinez-Maldonado, editors. Vol. 4B, pp. 165–193. Plenum Press, New YorkGoogle Scholar
  8. Boulpaep, E.L., Sackin, H. 1980. Electrical analysis of intraepithelial barriers.In: Current Topics in Membranes and Transport. E.L. Boulpaep, editor. Vol. 13, pp. 169–197. Academic Press, New YorkGoogle Scholar
  9. Brown, R.H., Cohen, I., Noble, D. 1980. The interactions of protons, calcium and potassium ions on cardiac Purkinje fibers.J. Physiol. (London) 282:345–352Google Scholar
  10. Burckhardt, B.C., Frömter, E. 1980. Bicarbonate transport across the peribubular membrane of rat kidney proximal tubule.In: Hydrogen Ion Transport in Epithelia. I. Schulz, G. Sachs, J.G. Forte, and K.J. Ullrich, editors. pp. 277–286. Elsevier/North Holland, Amsterdam-New York-OxfordGoogle Scholar
  11. Cemerikić, D., Wilcox, C.S., Giebisch, G. 1982. Intracellular potential and K+ activity in rat kidney proximal tubular cells in acidosis and K+ depletion.J. Membrane Biol. 69:159–165Google Scholar
  12. Edelman, A., Bouthier, M., Anagnostopoulos, T. 1981. Chloride distribution in the proximal convoluted tubule ofNecturus kidney.J. Membrane Biol. 62:7–17Google Scholar
  13. Edelman, A., Curci, S., Samarzija, I., Frömter, E. 1978. Determination of intracellular K+ activity in rat kidney proximal tubular cells.Pfluegers Arch. 378:37–45Google Scholar
  14. Eisenberg, R.S., Gage, P.W. 1969. Ionic conductances of the surface and transverse tubular membranes of frog sartorius fibers.J. Gen. Physiol. 53:279–297Google Scholar
  15. Frömter, E., Sato, K. 1976. Electrical events in active H+/HCO3− transport across rat kidney proximal tubular epithelium.In: Gastric Hydrogen Ion Secretion. D.K. Kasbekar, G. Sachs, and W.S. Rehm, editors. pp. 382–403. Dekker, New YorkGoogle Scholar
  16. Frömter, E., Sato, K., Gessner, K. 1975. Acetazolamide inhibits passive buffer exit from rat kidney proximal tubular cells.Pfluegers Arch. 359:R118Google Scholar
  17. Fujimoto, M., Kubota, T. 1976. Physicochemical properties of a liquid ion exchanger microelectrode and its application to biological fluids.Jpn. J. Physiol. 26:631–650Google Scholar
  18. Giebisch, G., Malnic, G., DeMello, G.B., DeMello-Aires, M. 1977. Kinetics of luminal acidification in cortical tubules of the rat kidney.J. Physiol. (London) 267:571–599Google Scholar
  19. Giebisch, G., Sullivan, L.P., Whittembury, G. 1973. Relationship between tubular net sodium reabsorption and peritubular potassium uptake in the perfusedNecturus kidney.J. Physiol. (London) 250:51–74Google Scholar
  20. Guggino, W.B., Boulpaep, E.L., Giebisch, G. 1982a. Electrical properties of chloride transport across theNecturus proximal tubule.J. Membrane Biol. 65:185–196Google Scholar
  21. Guggino, W.B., London, R., Boulpaep, E.L., Giebisch, G. 1982b. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium.J. Membrane Biol. (in press).Google Scholar
  22. Kubota, T., Biagi, B.A., Giebisch, G. 1983. Intracellular potassium activity measurements in single proximal tubules ofNecturus kidney.J. Membrane Biol. (in press) Google Scholar
  23. Malnic, G., Mello-Aires, M., Giebisch, G. 1971. Potassium transport across renal distal tubules during acid-base disturbances.Am. J. Physiol. 221:1192–1208Google Scholar
  24. Matsumura, Y., Guggino, W.B., Giebisch, G. 1982. Electrical effects of K+ and HCO3− on proximal tubule cells ofNecturus: Intracellular K+ affects K+ conductance.Kidney Int. 21:281A. (Abstr.)Google Scholar
  25. Miller, R.B., Tyson, I., Relman, A.S. 1963. pH of isolated resting skeletal muscle and its relation to potassium content.Am. J. Physiol. 204:1048–1054Google Scholar
  26. Sackin, H., Boulpaep, E.L. 1981. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport.Am. J. Physiol. 241:F540-F555Google Scholar
  27. Shindo, T., Spring, K.R. 1981. Chloride movement across the basolateral membrane of proximal tubule cells.J. Membrane Biol. 58:35–42Google Scholar
  28. Simmons, D.H., Avedon, M. 1959. Acid-base alterations and plasma potassium concentration.Am. J. Physiol. 197:319–326Google Scholar
  29. Spring, K.R., Kimura, G. 1978. Chloride reabsorption by renal proximal tubules ofNecturus.J. Membrane Biol. 38:233–254Google Scholar
  30. Stanton, B.A., Giebisch, G. 1982. Effects of pH on K+ transport by renal distal tubule.Am. J. Physiol. 241:F544-F551Google Scholar
  31. Steels, P.S., Boulpaep, E.L. 1976. Effect of pH on ionic conductances of the proximal tubule epithelium ofNecturus and the role of buffer permeability.Fed. Proc. 35:465 (Abstr.)Google Scholar
  32. Struyvenberg, A., Morrison, R.B., Relman, A.S. 1968. Acid-base behavior of separated canine renal tubule cells.Am. J. Physiol. 214:1155–1162Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Takahiro Kubota
    • 1
  • Bruce A. Biagi
    • 1
  • Gerhard Giebisch
    • 1
  1. 1.Department of PhysiologyYale University School of MedicineNew Haven

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