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Membranous localization and properties of ATPase of rat liver lysosomes

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Summary

Lysosomes isolated from rat liver were found to have ATPase activity (EC No. 3.6.1.3). Subfractionation of the lysosomes revealed a membranous localization of ATPase activity. The enzyme has half maximal activity at 0.2mm ATP and is inhibited by high concentrations of ATP. The apparentK m for divalent metal is 0.2mm, and either ca2+ or Mg2+ give maximal activity.

The ATPase activity has latency when lysosomes are isolated from rats treated with Triton WR-1339. This latency may be due to the presence of internalized sucrose because the activity ofL fraction lysosomes is much less latent and Triton WR-1339 itself is not inhibitory. The latency of glucosamindase, a marker enzyme for lysosomes, contrasts with the low latency of the ATPase and points to an ATPase with an exposed active site in intact lysosomes.

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Schneider, D.L. Membranous localization and properties of ATPase of rat liver lysosomes. J. Membrain Biol. 34, 247–261 (1977). https://doi.org/10.1007/BF01870302

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Keywords

  • Sucrose
  • Human Physiology
  • ATPase Activity
  • Maximal Activity
  • Divalent Metal