Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Membranous localization and properties of ATPase of rat liver lysosomes

  • 27 Accesses

  • 22 Citations


Lysosomes isolated from rat liver were found to have ATPase activity (EC No. Subfractionation of the lysosomes revealed a membranous localization of ATPase activity. The enzyme has half maximal activity at 0.2mm ATP and is inhibited by high concentrations of ATP. The apparentK m for divalent metal is 0.2mm, and either ca2+ or Mg2+ give maximal activity.

The ATPase activity has latency when lysosomes are isolated from rats treated with Triton WR-1339. This latency may be due to the presence of internalized sucrose because the activity ofL fraction lysosomes is much less latent and Triton WR-1339 itself is not inhibitory. The latency of glucosamindase, a marker enzyme for lysosomes, contrasts with the low latency of the ATPase and points to an ATPase with an exposed active site in intact lysosomes.

This is a preview of subscription content, log in to check access.


  1. 1.

    Ahkong, A.F., Fisher, D., Tampion, W., Lucy, J.A. 1975. Mechanisms of cell fusion.Nature (London) 253:194

  2. 2.

    Amar-Costesec, A., Wibo, M., Thines-Sempoux, D., Beaufay, H., Berthet, J. 1974. Analytical study of microsomes and isolated subcellular membranes from rat liver. IV. Biochemical, physical, and morphological modifications of microsomal components induced by digitonin, EDTA, and pyrophosphate.J. Cell Biol. 62:717

  3. 3.

    Banks, P. 1965. The ATPase of chromaffin granules.Biochem. J. 95:490

  4. 4.

    Chen, P.S., Toribara, T.Y., Warner, H. 1956. Microdetermination of phosphorus.Anal. Chem. 28:1756

  5. 5.

    Contessa, A.R., Bruni, A. 1971. Different effects of oligomycin and dicyclohexylcarbodiimide on ATPases from mammalian cells.Biochim. Biophys. Acta 241:334

  6. 6.

    Dean, R.T. 1975. Direct evidence of importance of lysosomes in degradation of intracellular proteins.Nature (London) 257:414

  7. 7.

    Dormer, R.L., Ashcroft, S.J.H. 1974. Studies on the role of calcium ions in the stimulation by adrenaline of amylase release from rat parotid.Biochem. J. 144:543

  8. 8.

    Duve, C. de 1963. The lysosome concept.In: Lysosomes, Ciba Foundation Symposium. A.V.S. de Reuck and M.P. Cameron, editors. p. 1. Little-Brown, Boston

  9. 9.

    Duve, C. de, Barsy, T. de, Poole, B., Trouet, A., Tulkens, P., Van Hoof, F. 1974. Lysomotrophic agents.Biochem. Pharmacol. 23:2495

  10. 10.

    Duve, C. de, Pressman, B.C., Gianetto, R., Wattriaux, R., Appelmans, F. 1955. Tissue fractionation studies. Intracellular distribution patterns of enzymes in rat-liver tissue.Biochem. J. 60:604

  11. 11.

    Duve, C. de, Wattiaux, R. 1966. Functions of lysosomes.Annu. Rev. Physiol. 28:435

  12. 12.

    Eibl, H., Lands, W.E.M. 1969. A new, sensitive determination of phosphate.Anal. Biochem. 30:51

  13. 13.

    Evans, W.H., Kremmer, T., Culvenor, J.G. 1976. Role of membrane in bile formation.Biochem. J. 154:589

  14. 14.

    Findlay, J., Levvy, G.A., Marsch, C.A. 1958. Inhibition of glycosidases by aldonolactones of corresponding configuration. 2. Inhibitors of β-N-acetylglucosaminidase,Biochem. J. 69:467

  15. 15.

    Garnett, H.M., Kemp, R.B. 1975. Calcium-and magnesium-activated ATPases in the plasma membrane of mouse liver cells.Biochim. Biophys. Acta 382:526

  16. 16.

    Goldberg, A.L., Dice, J.F. 1974. Intracellular protein degradation in mammalian and bacterial cells.Annu. Rev. Biochem. 43:835

  17. 17.

    Goldstein, J.L., Brunschede, G.Y., Brown, M.S. 1975. Inhibition of proteolytic degradation of LDL in human fibroblasts by chloroquine concanavalin A and Triton WR-1339.J. Biol. Chem. 250:7854

  18. 18.

    Guggenheim, E.A. 1926. The determination of the velocity constant of a unimolecular reaction.Philos. Mag. 2:538

  19. 19.

    Hellman, B. 1975. The effect of calcium and divalent ionophores on the stability of isolated secretory granules.FEBS Lett. 54:343

  20. 20.

    Iritani, N., Wells, W.W. 1974. Studies on a bicarbonate stimulated ATPase and carbonic anhydrase system in rat liver lysosomes.Arch. Biochem. Biophys. 164:357

  21. 21.

    Jacques, P.J. 1968. Thesis “Epuration plasmatique de proteines etrangeres. Leur capture et leur destinee l'appareil vacuolaire du foie” Librairie Universitaire, Louvain, Belgium

  22. 22.

    Kagawa, Y. 1974. Dissociation and reassembly of the inner mitochondrial membrane.In: Methods in Membrane Biology. E.D. Korn, editor. p. 201. Plenum, New York

  23. 23.

    Katz, S., Glostein, R. 1975. Calcium-stimulated membrane phosphorylation and ATPase activity of the human erythrocyte.Biochim. Biophys. Acta 389:314

  24. 24.

    Kaulen, H.D., Henning, R., Stöffel, W. 1970. Comparison of some enzymes of the lysosomal and the plasma membrane of the rat liver cell.Hoppe-Seyler's Z. Physiol. Chem. 351:1555

  25. 25.

    Lansman, J., Haynes, D.H. 1975. Kinetics of a calcium-triggered membrane aggregation reaction of phospholipid membranes.Biochim. Biophys. Acta 394:335

  26. 26.

    Leighton, F., Poole, B., Beaufay, H., Baudhuin, P., Coffey, J.W., Fowler, S., Duve, C. de 1968. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR-1339.J. Cell Biol. 37:483

  27. 27.

    Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:263

  28. 28.

    Maeda, T., Ohnishi, S. 1974. Membrane fusion. Transfer of phospholipid molecules between phospholipid bilayer membranes.Biochem. Biophys. Res. Commun. 60:1509

  29. 29.

    Mego, J.L., Farb, R.M., Barnes, J. 1972. An ATP-dependent stabilization of proteolytic activity in heterolysosomes.Biochem. J. 128:763

  30. 30.

    Miller, C., Racker, E. 1976. Fusion of phospholipid vesicles reconstituted with cytochromec oxidase and mitochondrial hydrophobic protein.J. Membrane Biol. 26:319

  31. 31.

    Neely, A.N., Mortimore, G.E. 1974. Localization of products of endogenous proteolysis in lysosomes of perfused rat liver.Biochem. Biophys. Res. Commun. 59:680

  32. 32.

    Papahadjopoulos, D., Poste, G., Schaeffer, D.E., Vail, W.J. 1974. Membrane fusion and molecular segregation in phospholipid vesicles.Biochim. Biophys. Acta 325:10

  33. 33.

    Peretz, H., Toister, Z., Laster, Y., Loyter, A. 1974. Fusion of intact human erythrocytes and erythrocyte ghosts.J. Cell Biol. 63:1

  34. 34.

    Plattner, H., Fuchs, S. 1975. X-ray microanalysis of calcium binding sites inParamecium. With special reference to exocytosis.Histochemistry 45:23

  35. 35.

    Poste, G., Allison, A.C. 1973. Membrane fusion.Biochim. Biophys. Acta 300:421

  36. 36.

    Racker, E., Burstein, C., Loyter, A., Christiansen, R.O. 1970. The sidedness of the inner mitochondrial membrane.In: Electron Transport and Energy Conservation. J.M. Tager, S. Papa, E. Quagliariello and E.C. Slater, editors. p. 235. Adriatica Editrice, Bari

  37. 37.

    Ray, T.K. 1970. A modified method for the isolation of the plasma membrane from rat liver.Biochim. Biophys. Acta 196:1

  38. 38.

    Robblee, L.S., Shepro, D. 1976. The effect of external calcium and lanthanum on platelet calcium content and on the release reaction.Biochim. Biophys. Acta 436:448

  39. 39.

    Schimke, R.T. 1974. Turnover of membrane proteins in animal tissues.In: Biochemistry of Cell Walls and Membranes. C.F. Fox, editor. p. 229. University Park Press, Baltimore

  40. 40.

    Schneider, D.L. 1974. A membranous ATPase unique to lysosomes.Biochem. Biophys. Res. Commun. 61:882

  41. 41.

    Simon, F.R., Arias, I.M. 1973. Alteration of bile canalicular enzymes in cholestasis.J. Clin. Invest. 52:765

  42. 42.

    Simpson, M.V. 1953. The release of labeled amino acids from the proteins of rat liver slices.J. Biol. Chem. 201:143

  43. 43.

    Thines-Sempoux, D. 1973. A comparison between the lysosomal and plasma membrane.In: Lysosomes in Biology and Pathology. J.T. Dingle, editor. Vol. 3, p. 278. American Elsevier, New York

  44. 44.

    Trouet, A. 1964. Immunisation de lapins par des lysosomes hepatiques de rats traites au Triton WR-1339.Arch. Intern. Physiol. Biochim. 72:698

  45. 45.

    Wattiaux-de Coninck, S., Wattiaux, R. 1969. Nucleoside diphosphatase in purified preparations of rat liver lysosomes.FEBS Lett. 5:355

  46. 46.

    Wisher, M.H., Evans, W.H. 1975. Functional polarity of the rat hepatocyte surface membrane.Biochem. J. 146:375

  47. 47.

    Woodin, A.M., Wieneke, A.A. 1964. The participation of calcium, ATP and ATPase in the extrusion of the granule proteins from the polymorphonuclear leukocyte.Biochem. J. 90:498

  48. 48.

    Zabucchi, G., Soranzo, M.R., Rossi, F., Romeo, D. 1975. Exocytosis in human PMN leukocytes induces by A23187 and calcium.FEBS Lett. 54:44

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Schneider, D.L. Membranous localization and properties of ATPase of rat liver lysosomes. J. Membrain Biol. 34, 247–261 (1977). https://doi.org/10.1007/BF01870302

Download citation


  • Sucrose
  • Human Physiology
  • ATPase Activity
  • Maximal Activity
  • Divalent Metal