Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics

Summary

Phosphatidylcholine was extracted from egg yolks and sonicated withn-alkyl alcohols to give vesicles. The magnetic environment of the external compartment was then modified by the addition of shift reagent (Co2+). This allowed determination of the average lifetime for exchange of water molecule protons by observing the relaxation rate dependence on the interval between pulses in a Carr-Purcell sequence. For vesicles containingn-hexanol as 33% of lipid, the average water molecule lifetime in the internal compartment was found to be substantially less than that for a vesicle without hexanol. The lifetime, 11.3±4 msec, is equivalent to a permeability coefficient of 60 μ/sec at 34°C.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Allerhand, A., Gutowsky, H.S. 1964. Spin echo NMR studies of chemical exchange.J. Chem. Phys. 41:2115

  2. 2.

    Andrasko, J., Forsen, S. 1974. NMR study of rapid water diffusion across lipid bilayers in dipalmitoyl lecithin vesicles.Biochem. Biophys. Res. Commun. 60:813

  3. 3.

    Bangham, A.D., DeGier, J., Greville, G.O. 1967. Osmotic properties and water permeability of phospholipid crystals.Chem. Phys. Lipids 1:225

  4. 4.

    Bangham, A.D., Hill, M.W., Miller, N. 1974. Preparation and use of Liposomes as models of biological membranes.Methods Membr. Biol. 1:1

  5. 5.

    Bangham, A.D., Standish, N.M., Miller, N. 1965. Cation permeability of phospholipid membranes: Effect of narcotics.Nature (London) 208:1295

  6. 6.

    Cass, A., Finkelstein, A. 1967. Water permeability of thin lipid membranes.J. Gen. Physiol 50:1765

  7. 7.

    Farrar, T.C., Becker, E.D. 1971. Pulse and Fourier Transform NMR. Academic Press, New York

  8. 8.

    Gent, M.P.N., Prestegard, J.H. 1974. Cholesterol-phosphatidylcholine interactions in vesicle systems.Biochemistry 13:4027

  9. 9.

    Huang, C. 1969. Studies on phosphatidylcholine vesicles. Formation and physical characteristics.Biochemistry 8:344

  10. 10.

    Levine, Y.K. 1972. Physical studies of membrane structure.Prog. Biophys. Mol. Biol. 24:3

  11. 11.

    Papahadjapoulos, D., Kimelberg, H.K. 1973. Phospholipid vesicles as models for biological membranes.Prog. Surf. Sci. 4(II):141

  12. 12.

    Reeves, J.P., Dowben, R.M. 1970. Water permeability of phospholipid vesicles.J. Membrane Biol. 3:123

  13. 13.

    Singleton, W.S., Gray, M.S., Brown, M.L., White, J.L. 1965. Chromatographically homogeneous lecithin from egg phospholipids.J. Am. Oil. Chem. Soc. 42:53

  14. 14.

    Thompson, T.E., Henn, F.A. 1970. Experimental phospholipid model membranes.In: Membranes of Mitochondria and Chloroplasts. E. Racker, editor. pp. 1–52. Van Nostrand and Reinhold, New York

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hochster, H.S., Prestegard, J.H. Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics. J. Membrain Biol. 35, 303–307 (1977). https://doi.org/10.1007/BF01869955

Download citation

Keywords

  • Phosphatidylcholine
  • Relaxation Rate
  • Water Permeability
  • Permeability Coefficient
  • Hexanol