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Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics


Phosphatidylcholine was extracted from egg yolks and sonicated withn-alkyl alcohols to give vesicles. The magnetic environment of the external compartment was then modified by the addition of shift reagent (Co2+). This allowed determination of the average lifetime for exchange of water molecule protons by observing the relaxation rate dependence on the interval between pulses in a Carr-Purcell sequence. For vesicles containingn-hexanol as 33% of lipid, the average water molecule lifetime in the internal compartment was found to be substantially less than that for a vesicle without hexanol. The lifetime, 11.3±4 msec, is equivalent to a permeability coefficient of 60 μ/sec at 34°C.

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Hochster, H.S., Prestegard, J.H. Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics. J. Membrain Biol. 35, 303–307 (1977). https://doi.org/10.1007/BF01869955

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  • Phosphatidylcholine
  • Relaxation Rate
  • Water Permeability
  • Permeability Coefficient
  • Hexanol