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The Journal of Membrane Biology

, Volume 2, Issue 1, pp 71–84 | Cite as

An ultrastructural study of isolated rat skeletal muscle mitochondria in various metabolic states

  • Jerry M. Kuner
  • Robert E. Beyer
Article

Summary

An electron microscopic examination was made of isolated rat skeletal muscle mitochondria in various functional states. Shifts in the inner membrane ultrastructure of populations of mitochondria were observed under certain conditions. However, ultrastructural transformations reported by others during rapid changes in biochemical states were not observed in skeletal muscle mitochondria. There does not appear to be a strict correlation between metabolic states and ultrastructural states in isolated rat skeletal muscle mitochondria as has been observed in isolated mitochondria from several other tissue types.

Keywords

Skeletal Muscle Human Physiology Functional State Microscopic Examination Tissue Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Chance, B., Williams, G. R. 1956. The respiratory chain and oxidative phosphorylation.In: Advances in Enzymology, F. F. Nord, editor, p. 65. Interscience, New York.Google Scholar
  2. 2.
    Estabrook, R. W. 1967. Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios.In: Methods in Enzymology, Vol. 10. R. W. Estabrook and M. E. Pullman, editors, p. 41. Academic Press, New York.Google Scholar
  3. 3.
    Gornall A. G., Bardawill, C. J., David, M. M. 1949. Determination of serum proteins by means of the biuret reaction.J. Biol. Chem. 177:751.Google Scholar
  4. 4.
    Goyer, R. A., Krall, R. 1969. Ultrastructural transformation in mitochondria isolated from kidneys of normal and lead intoxicated rats.J. Cell Biol. 41:393.Google Scholar
  5. 5.
    Green, D. E., Asai, J., Harris, R. A., Penniston, J. T. 1968. Conformational basis of energy transformations in membrane systems. III. Configurational changes in the mitochondrial inner membrane induced by changes in functional statesArch. Biochem. Biophys. 125:684.Google Scholar
  6. 6.
    Hackenbrock, C. R. 1966. Ultrastructural bases for metabolically linked mechanical activity in mitochondria. I. Reversible ultrastructural changes with change in metabolic steady state in isolated liver mitochondria.J. Cell Biol. 30:269.Google Scholar
  7. 7.
    — 1968. Ultrastructural bases for metabolically linked mechanical activity in mitochondria. II. Electron transport-linked ultrastructural transformations in mitochondria.J. Cell Biol. 37:345.Google Scholar
  8. 8.
    Harris, R. A., Penniston, J. T., Asai, J., Green, D. E. 1968. The conformational basis of energy conservation in membrane systems. II. Correlation between conformational change and functional states.Proc. Nat. Acad. Sci. 59:830.Google Scholar
  9. 9.
    —, Williams, C. H., Caldwell, M., Green, D. E., Valdivia, E. 1969. Energized configurations of heart mitochondriain situ.Science 165:700.Google Scholar
  10. 10.
    Jasper, D. K., Bronk, J. R. 1968. Studies on the physiological and structural characteristics of rat intestinal mucosa.J. Cell Biol. 38:277.Google Scholar
  11. 11.
    Makinen, M. W., Lee, C.-P. 1968. Biochemical studies of skeletal muscle mitochondria. I. Microanalysis of cytochrome content, oxidative and phosphorylative activities of mammalian skeletal muscle mitochondria.Arch. Biochem. Biophys. 126:75.Google Scholar
  12. 12.
    Millonig, G. 1961. A modified procedure for lead staining of thin sections.J. Biophys. Biochem. Cytol. 11:736.Google Scholar
  13. 13.
    Penniston, J. T., Harris, R. A., Asai, J., Green, D. E. 1968. The conformational basis of energy transformations in membrane systems. I. Conformational changes in mitochondria.Proc. Nat. Acad. Sci. 59:624.Google Scholar
  14. 14.
    Pullman, M. E., Schatz, G. 1967. Mitochondrial oxidations and energy coupling.Ann. Rev. Biochem. 36:539.Google Scholar
  15. 15.
    Sordahl, L. A., Blailock, Z. R., Kraft, G. H., Schwartz, A. 1969. The possible relationship between ultrastructure and biochemical state of heart mitochondria.Arch. Biochem. Biophys. 132:404.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1970

Authors and Affiliations

  • Jerry M. Kuner
    • 1
  • Robert E. Beyer
    • 1
  1. 1.Laboratory of Chemical Biology and Department of ZoologyUniversity of MichiganAnn Arbor

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