The Journal of Membrane Biology

, Volume 31, Issue 1, pp 205–219 | Cite as

Electrical properties and active solute transport in rat small intestine

I. Potential profile changes associated with sugar and amino acid transports
  • Yasunobu Okada
  • Wakoh Tsuchiya
  • Akihiko Irimajiri
  • Akira Inouye
Article

Summary

Addition ofd-glucose to the mucosal fluid resulted in a significant depolarization of the mucosal membrane potential (V m) in rat duodenum, jejunum, and ileum accompanied by an increase in the transepithelial potential difference (PD t). On the other hand,l-glucose did not inducePD t andV m changes. Glycine applied from the mucosal side also inducedV m-depolarization andPD t-increment in the ileum. Phlorizin added to the mucosal fluid or ouabain added to the serosal fluid inhibited the sugar-dependent changes inPD t andV m.

According to the analysis with an equivalent circuit model for the epithelium, it was concluded that an actively transported solute induced not only a depolarization of the mucosal (brush border) membrane but also a hyperpolarization of the serosal (baso-lateral) membrane of an epithelial cell, so that the origin of solute-inducedPD t changes should be attributed to changes in emf's at both membranes. The hyperpolarization of the serosal membrane in the presence of an actively transported solute was attributed to a mechanism of serosal electrogenic sodium pump stimulated by the increase in the extrusion rate of Na+ co-transported into the cell with sugar or amino acid.

Keywords

Ouabain Mucosal Membrane Phlorizin Transepithelial Potential Difference Rabbit Ileum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1977

Authors and Affiliations

  • Yasunobu Okada
    • 1
  • Wakoh Tsuchiya
    • 1
  • Akihiko Irimajiri
    • 1
  • Akira Inouye
    • 1
  1. 1.Department of PhysiologyKyoto University School of MedicineKyotoJapan

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