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The Journal of Membrane Biology

, Volume 116, Issue 3, pp 227–238 | Cite as

Bradykinin-induced potassium current in cultured bovine aortic endothelial cells

  • Margaret Colden-Stanfield
  • William P. Schilling
  • Lourival D. Possani
  • Diana L. Kunze
Articles

Summary

Bovine aortic endothelial cells (BAECs) respond to bradykinin with an increase in cytosolic-free Ca2+ concentration, [Ca2+] i , accompanied by an increase in surface membrane K+ permeability. In this study, electrophysiological measurement of K+ current was combined with86Rb+ efflux measurements to characterize the K+ flux pathway in BAECs. Bradykinin- and Ca2+-activated K+ currents were identified and shown to be blocked by the alkylammonium compound, tetrabutylammonium chloride and by the scorpion toxin,noxiustoxin, but not by apamin or tetraethylammonium chloride. Whole-cell and single-channel current analysis suggest that the threshold for Ca2+ activation is in the range of 10 to 100nm [Ca2+] i . The whole-cell current measurement show voltage sensitivity only at the membrane potentials more positive than 0 mV where significant current decay occurs during a sustained depolarizing pulse. Another K+ current present in control conditions, an inwardly rectifying K+ current, was blocked by Ba2+ and was not affected bynoxiustoxin or tetrabutylammonium chloride. Efflux of86Rb from BAEC monolayers was stimulated by both bradykinin and ionomycin. Stimulated efflux was blocked by tetrabutyl- and tetrapentyl-ammonium chloride and bynoxiustoxin, but not by apamin or furosemide. Thus,86Rb+ efflux stimulated by bradykinin and ionomycin has the same pharmacological sensitivity as the bradykinin- and Ca2+-activated membrane currents. The results confirm that bradykinin-stimulated86Rb+ efflux occurs via Ca2+-activated K+ channels. The blocking agents identified may provide a means for interpreting the role of the Ca2+-activated K+ current in the response of BAECs to bradykinin.

Key Words

endothelial cells K+ channels 86Rb+ efflux bradykinin cytosolic-free Ca2+ noxiustoxin 

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Margaret Colden-Stanfield
    • 1
  • William P. Schilling
    • 1
  • Lourival D. Possani
    • 1
  • Diana L. Kunze
    • 1
  1. 1.Department of Molecular Physiology and BiophysicsBaylor College of MedicineHouston

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