Some studies on lipid peroxidation in monomolecular and bimolecular lipid films
Hydrogen peroxide generated from dissolved oxygen through the alloxandialuric acid cycle affected both the permeability and the stability of lipid bilayer membranes. The permeability of the artificial membranes varied directly with the hydrogen peroxide concentration. Membrane stability varied inversely with the hydrogen peroxide concentration. Bilayers formed from solutions containing both phospholipid and the antioxidant vitamin E were less permeable and more stable in the presence of hydrogen peroxide than bilayers generated from solutions containing phospholipid alone. Peroxidation of phospholipid monolayers caused first an expansion of the films presumably through the introduction of peroxide groups. Further oxidation of phospholipid monolayers led to contraction of the films presumably through the formation of water-soluble products. The results of the monolayer studies and a consideration of the possible kinetics for the peroxidation reaction sequence have been used to explain the changes in the permeability and the stability of lipid bilayer membranes. Our data suggest that oxidation of lipid in biological membranes may first increase membrane permeability and then decrease membrane stability.
KeywordsLipid Peroxide Lipid Peroxidation Human Physiology Membrane Permeability
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- 1.Adam, N. K. 1926. The structure of thin films. VIII. Expanded films.Proc. Roy. Soc. (London) A 112:362.Google Scholar
- 2.Barber, A. A., Bernheim, F. 1967. Lipid peroxidation: Its measurement, occurrence and significance in animal tissues.In: Advances in Gerontological Research. B. L. Strehler, editor. Vol. 2, p. 355. Academic Press Inc., New York, London.Google Scholar
- 6.Deamer, D. W., Heikkila, R. E., Panganamala, R. V., Cohen, G., Cornwell, D. G. 1971. The alloxan-dialuric acid cycle and the generation of hydrogen peroxide.Physiol. Chem. Phys. 3:426.Google Scholar
- 8.Gutfreund, H. 1972. Enzymes: Physical Principles. p. 123. Wiley-Interscience, London.Google Scholar
- 10.Heikkila, R. E., Mezick, J. A., Cornwell, D. G. 1971. Destruction of specific membrane phospholipids during peroxidative hemolysis of vitamin E deficient erythrocytes.Physiol. Chem. Phys. 3:93.Google Scholar
- 12.Hughes, A. H., Rideal, E. K. 1933. On the rate of oxidation of monolayers of unsaturated fatty acids.Proc. Roy. Soc. (London) A 140:253.Google Scholar
- 13.Mezick, J. A., Settlemire, C. T., Brierley, G. P., Barefield, K. P., Jensen, W. N., Cornwell, D. G. 1971. Erythrocyte membrane interactions with menadione and the mechanism of menadione-induced hemolysis.Biochim. Biophys. Acta 219:361.Google Scholar
- 14.Packer, L., Deamer, D. W., Heath., R. L. 1967. Regulation and deterioration of structure in membranes.In: Advances in Gerontological Research. B. L. Strehler, editor. Vol. 2, p. 77. Academic Press Inc., New York, London.Google Scholar
- 17.Rothstein, A. 1970. Sulfhydryl groups in membrane structure and function.In: Current Topics in Membranes and Transport. F. Bronner and A. Kleinzeller, editors: Vol. 1, p. 170. Academic Press Inc., New York, London.Google Scholar