Technetium-99 (99Tc) is formed in significant amounts (6.2% fission yield) during fission in both nuclear reactors and nuclear bombs. The effects of technetium on soybeans (Glycine max) were studied in relation to (a) cytochemical events in the apical meristems of germinating seedlings; (b) growth responses to 0, 0.04, 0.2, 1.0, 5.0, and 20.0 ppm Tc; (c) growth responses to varying levels of Tc after a prior 5-day germination on Tc-free media, and (d) response to Tc in the presence of added manganese.
By 20 days, reductions in growth were evident at all levels of Tc except 0.04 ppm (Experiment 2). Root growth was most severely affected, and seedling abnormality at 20 ppm was fivefold greater than that of the controls. The effect of 20 ppm Tc was evident at 10 days when the accumulated absorption dose was approximately 25 rads. The first evidence of damage at this dose was a delay in the initiation of the first trifoliate leaf. The shoot meristem size was 1.2-fold smaller than that of the control; however, there was no cytological evidence of radiation-induced damage. Observation of mitotic figures did not reveal any chromosome aberrations, micronuclei, or chromosome bridges. The lowest level of Tc showing toxicity was 0.2 ppm which resulted in a 31% reduction in growth at 20 days. The accumulated dose was 0.5 rad (0.025 rad/day) and thus it seems unlikely that the rapid inhibition of growth and development is due to radiological toxicity. It is quite probable that the growth effects are due to chemical toxicity possibly due to nutrient competition and/or substitution in uptake or metabolism. However, extremely low doses of radiation have been shown to delay the onset of DNA synthesis (possibly by membrane effects) inTradescantia and until the actual mechanism of Tc inhibition is determined a radiation effect cannot be totally ruled out.
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Baumann, E. J., N. Z. Searle, A. A. Talow, E. Siegel, and S. M. Seidlin. 1956. Behavior of thyroid toward elements of the seventh periodic group.American Journal of Physiology 185: 71–76.
Beasley, R. M., H. E. Palmer, and W. B. Nelp. 1966. Distribution and excretion of technitium in humans.Health Physics 12: 1425–1435.
Berlyn, G. P. 1967. The structure of germination inPinus lambertiana. Bulletin 71. Yale University School of Forestry. New Haven, Connecticut.
Berlyn, G. P., and J. P. Miksche. 1976.Botanical microtechnique and cytochemistry. Iowa State University Press, Ames, Iowa.
Cataldo, D. A., and G. P. Berlyn. 1974. An evaluation of selected physical characteristics and metabolism of enzymatically separated mesophpyll cells and minor veins of tobacco.American Journal of Botany 61: 957–963.
Cataldo, D. A., R. E. Wildung, and T. R. Garland. 1974. Accumulation of technetium from soil by plants: II. Potential mechanisms for uptake and toxicity. Report BNWL—1950 PT2. Battelle Pacific Northwest Laboratory, Richland, Washington.
Coryell, C. D., and N. Sugarman. 1951. The radioactivity of the fission products, InNational Nuclear Energy Series. Manhattan Project, technical section. Div. LI. Vol. 9. Book 2, part V, pp. 517–548.
Friedlander, G., J. W. Kennedy, and J. M. Miller, 1964.Nuclear and radiochemistry. Wiley, New York.
Hill, D. L., and J. A. Wheeler. 1953. Nuclear constitution and the interpretation of fission phenomena.Physics Review 89: 1102.
Kotegov, K. V., O. N. Pavlov, and V. P. Shvedov. 1968. Technetium. InAdvances in inorganic chemistry and radiochemistry. Academic Press, New York. pp. 1–9.
Landa, E. R., L. J. Hart, and R. G. Gast. 1975. Uptake and distribution of technetium-99 in higher plants. Unpublished paper. Department of Soil Science, University of Minnesota, St. Paul.
Leblond, C. P., B. Kopriwa, and B. Messier. 1963. Radioautography as a histochemical tool. In R. Wegman (Ed.),Histochemistry and Cytochemistry. Macmillan, New York. Pp. 1–31.
Miksche, J. P. 1961. Developmental vegetative morphology ofGlycine max.Argronomy Journal 53: 121–128.
Wildung, R. E., T. R. Garland, and D. A. Cataldo. 1974a. Preliminary studies on the uptake of technetium by soybeans. Report BNWL-1950 PT 2, Battelle Pacific Northwest Laboratory, Richland, Washington.
Wildung, R. E., D. A. Cataldo, and T. R. Garland. 1974b. Accumulation of technetium from soil by plants: I. Uptake of technetium from soil by soybeans and wheat. Report BNWL-1950 PT2, Battelle Pacific Northwest Laboratory, Richland, Washington.
This research was partially supported by ERDA Contract EY-76-S-02-4139
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Berlyn, G.P., Dhillon, S.S. & Koslow, E.E. Technetium: A toxic waste product of the nuclear fuel cycle: Effects on soybean growth and development. Environmental Management 4, 149–156 (1980). https://doi.org/10.1007/BF01866511
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