Research in Experimental Medicine

, Volume 182, Issue 1, pp 71–78

Identification of glucocorticoid receptors in normal and neoplastic adult human lung

  • G. Hochhaus
  • P. Rohdewald
  • H. Möllmann
  • D. Greschuchna
Original Contributions

Summary

A dexamethasone-binding protein was detected in cytosol of five normal and three neoplastic lung tissue samples. The apparent dissociation constant of the dexamethasone protein complex ranged from 5.8 to 21 nM (11.4±5.7 nM for normal tissue cytosol, 12.1±7.7 nM for the neoplastic tissue cytosol). The binding protein sedimented at 8 S in sucrose density gradient at low ionic strength and showed a high specifity for glucocorticoids. It was concluded that the dexamethasone-binding protein had the characteristics of a glucocorticoid receptor. The glucocorticoid receptor content was determined to be 12–77 fmol/mg protein (37.1±18.9 fmol/mg protein for the normal tissue cytosol, 51.9±23.9 fmol/mg protein for the neoplastic tissue cytosol).

Key words

Glucocorticoid receptor Adult human lung Lung cancer 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • G. Hochhaus
    • 1
  • P. Rohdewald
    • 1
  • H. Möllmann
    • 2
  • D. Greschuchna
    • 3
  1. 1.Institut für Pharmazeutische ChemieUniversität MünsterMünster i. Westf.Federal Republic of Germany
  2. 2.Ruhruniversität BochumMedizinische Universitätsklinik und Poliklinik “Bergmannsheil”BochumFederal Republic of Germany
  3. 3.Ruhrlandklinik der LVA RheinprovinzEssenFederal Republic of Germany

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