Research in Experimental Medicine

, Volume 181, Issue 2, pp 113–123 | Cite as

Influence of dietary purines on pool size, turnover, and excretion of uric acid during balance conditions

Isotope Studies Using15N-Uric Acid
  • W. Löffler
  • W. Gröbner
  • R. Medina
  • N. Zöllner
Original Contributions


Pool size, turnover, and excretion of uric acid were investigated in three normal subjects both during purine-free, isoenergetic liquid formula diet and during additional purine administration by use of isotope dilution techniques. The fractional turnover of the uric acid pool was increased during dietary purine administration suggesting an increased total body uric acid clearance as a result of the increase in renal clearance. Fractional turnover increased more in the female subject than in males, while pool size was increased less. It can be calculated from the results obtained that endogenous uric acid synthesis is not inhibited by dietary purines.

Key words

Uric acid, pool size, turnover, excretion Dietary purines Isotope dilution 


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  1. Benedict JD, Forsham PH, Stetten D (1949) The metabolism of uric acid in the normal and gouty human studied with the aid of isotopic uric acid. J Biol Chem 181:183–193PubMedGoogle Scholar
  2. Benedict JD, Forsham PH, Roche M, Soloway S, Stetten D (1950) The effect of salicylates and adrenocorticotropic hormone upon the miscible pool of uric acid in gout. J Clin Invest 29:1104–1111PubMedGoogle Scholar
  3. Bishop Ch, Garner W, Talbott JH (1951a) Pool size, turnover rate, and rapidity of equilibration of injected isotopic uric acid in normal and pathological subjects. J Clin Invest 30:879–888PubMedGoogle Scholar
  4. Bishop Ch, Rand R, Talbott JH (1951b) The effect of Benemid on uric acid metabolism in one normal and one gouty subject. J Clin Invest 30:889–895PubMedGoogle Scholar
  5. Bishop Ch, Beyer A, Talbott JH (1954) Isotopic uric acid in gouty and rheumatoid arthritis patients treated with probenecid and phenylbutazone. Proc Soc Exp Biol Med 86:760–762PubMedGoogle Scholar
  6. Bowering J, Calloway DH, Margen S, Kaufmann NA (1969) Dietary protein level and uric acid metabolism in normal man. J Nutr 100:249–261Google Scholar
  7. Brooks BA, Lant AF (1976) Chemical instability of [2-14C] uric acid in alkaline solution: the effect on observed kinetics of urate transport in human erythrocytes. Anal Biochem 75:640–645PubMedGoogle Scholar
  8. Buzard J, Bishop Ch, Talbott JH (1952) Recovery in humans of intravenously injected isotopic uric acid. J Biol Chem 196:179–184PubMedGoogle Scholar
  9. Chasson AL, Grady HJ, Stanley MA (1961) Determination of creatinine by means of automatic chemical analysis. Am J Clin Pathol 35:83–88Google Scholar
  10. Dosman JA, Crawhall JC, Klassen GA (1975) Uric acid kinetic studies in the immediate post-myocardial-infarction period. Metabolism 24:473–480PubMedGoogle Scholar
  11. Eisen AZ, Seegmiller JE (1961) Uric acid metabolism in psoriasis. J Clin Invest 40:1486–1494PubMedGoogle Scholar
  12. Folin O (1922) Laboratory manual of biological chemistry. McGraw-Hill, New YorkGoogle Scholar
  13. Geren W, Bendich A, Bodansky O, Brown GB (1950) The fate of uric acid in man. J Biol Chem 183:21–31Google Scholar
  14. Homes EW, McDonald JA, McCord JM, Wyngaarden JB, Kelley WN (1973) Human glutamine phosphoribosylpyrophosphate amidotransferase: Kinetic and regulatory properties. J Biol Chem 248:144–150PubMedGoogle Scholar
  15. Husdan H, Rapoport A (1968) Estimation of creatinine by the Jaffé reaction. A comparison of three methods. Clin Chem 14:222–238PubMedGoogle Scholar
  16. Johnson LA, Emmerson BT (1972) Isolation of crystalline uric acid from urine, for urate pool or turnover measurements. Clin Chim Acta 41:389–393PubMedGoogle Scholar
  17. Kelley WN, Rosenbloom FM, Seegmiller JE, Howell RR (1968) Excessive production of uric acid in type I glycogen storage disease. J Pediatr 72:488–496PubMedGoogle Scholar
  18. Löffler W, Gröbner W, Zöllner N (1981) Nutrition and uric acid metabolism: plasma level, turnover, excretion. Fortschr Urol Nephrol 16:8–18Google Scholar
  19. Praetorius E, Poulsen H (1953) Enzymatic determination of uric acid with detailed directions. Scand J Clin Lab Invest 5:273–280PubMedGoogle Scholar
  20. Ross J, Martin E (1970) A rapid procedure for preparing gas samples for nitrogen-15 determination. Analyst 95:817–821Google Scholar
  21. Scott JT, Holloway VP, Glass H, Arnot RN (1969) Studies of uric acid pool size and turnover rate. Ann Rheum Dis 28:366–373PubMedGoogle Scholar
  22. Seegmiller JE, Grayzel AJ, Laster L, Liddle L (1961) Uric acid production in gout. J Clin Invest 40:1304–1314PubMedGoogle Scholar
  23. Seegmiller JE, Klinenberg JR, Miller J, Watts RWE (1968) Suppression of glycine-15N incorporation into urinary uric acid by adenine-8-13C in normal and gouty subjects. J Clin Invest 47:1193–1203PubMedGoogle Scholar
  24. Sorensen LB (1959) Degradation of uric acid in man. Metabolism 8:687–703PubMedGoogle Scholar
  25. Sorensen LB (1960) The elemination of uric acid in man studied by means of C14-labelled uric acid. Scand J Clin Lab Invest 12: Suppl 54Google Scholar
  26. Sorensen LB (1962) The pathogenesis of gout. Arch Int Med 109:379–390Google Scholar
  27. Wyngaarden JB, Stetten D (1953) Uricolysis in normal man. J Biol Chem 203:9–21PubMedGoogle Scholar
  28. Wyngaarden JB (1955) The effect of phenylbutazone on uric acid metabolism in two normal subjects. J Clin Invest 34:256–262PubMedGoogle Scholar
  29. Zöllner N (1960) Moderne Gichtprobleme. Ätiologie, Pathogenese, Klinik. Ergeb Inn Med Kinderheilk 14:321–389Google Scholar
  30. Zöllner N (1963) Eine einfache Modifikation der enzymatischen Harnsäurebestimmung. Z Klin Chem Klin Biochem 1:178–182Google Scholar
  31. Zöllner N, Griebsch A, Gröbner W (1972) Einfluß verschiedener Purine auf den Harnsäurestoffwechsel. Ernährungsumschau 3:79–82Google Scholar
  32. Zöllner N, Gröbner W (1977) Dietary feedback regulation of purine and pyrimidine biosynthesis in man. Ciba Found Symp 48:165–178PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • W. Löffler
    • 1
  • W. Gröbner
    • 1
  • R. Medina
    • 2
  • N. Zöllner
    • 1
  1. 1.Medizinische PoliklinikUniversität MünchenMünchen 2Federal Republic of Germany
  2. 2.Lehrstuhl für allgemeine Chemie und BiochemieTechnischen Universität MünchenFreising-WeihenstephanFederal Republic of Germany

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