Research in Experimental Medicine

, Volume 169, Issue 3, pp 203–212 | Cite as

Makroskopische und mikromorphologische Befunde zur Beurteilung der Vitalität extrakorporal perfundierter Schweinelebern

  • M. Fischer
  • Th. Wustrow
Article
Received:

Zusammenfassung

Zur Beurteilung der Vitalität extrakorporal perfundierter Lebern gibt es zahlreiche biochemische Umsatzreaktionen. Bei therapeutischer Anwendung ist jedoch die Kenntnis rascher und einfacher Vitalitätsparameter wichtig. Anhand von 33 homologen Schweineleberperfusionen fanden wir, daß die sorgfältige makroskopische Beurteilung bei entsprechender Erfahrung einen guten Anhaltspunkt für den Funktionszustand der verwendeten Leber liefert. In Zusammenhang mit mikromorphologischen Untersuchungen, vor allem nach Perfusion mit Tusche, lassen sich daraus zuverlässige Anhaltspunkte für die Funktionsfähigkeit der Lebern ableiten.

Schlüsselwörter

Extrakorporale Leberperfusion Vitalitätsparameter Tusche-Gelatineperfusion Peripherer Widerstand der Leber 

Macroscopic and micro-morphologic evaluation of viability in extracorporeal perfused pig livers

Summary

Several biochemical reactions are used in evaluating viability of extracorporeal perfused livers. Simple and quick tests of organ function, however, are needed in therapeutic application in man.

In 33 homologous porcine liver perfusions we found that accurate observation of the macroscopic aspect gave a good criterion of organ function. The combination with micro-morphologic findings, especially after perfusion with Indian ink, proved to be a reliable base for evaluating the functional state of the livers used.

Key words

Extracorporeal liver perfusion Parameter of viability Indian ink and gelatine perfusion Peripheral resistance of the liver 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 1.
    ABOUNA, G.M., ASHCROFT, T., HULL, C., HODSON, A., KIRKLEY, J., WALDNER, D.N.: The assessment of function of the isolated perfused porcine liver. Br. J. Surg.56 289–295 (1969)PubMedGoogle Scholar
  2. 2.
    ABOUNA, G.M., ASHCROFT, T., JOUNG, J.R.: Transhepatie vascular resistance during prolonged isolated liver perfusion. Br. J. Surg.56 383–388 (1969)Google Scholar
  3. 3.
    ABOUNA, G.M., COOK, J.S., FISHER, L. Mc A., STILL, W.J., COSTA, G., HUME, D.M.: Treatment of acute hepatic coma by ex vivo baboon and human liver perfusions. Br. J. Surg.71 537–546 (1972)Google Scholar
  4. 4.
    BRAUER, R.W.: Liver Circulation and Function. Physiol. Rev.43 115–213 (1963)PubMedGoogle Scholar
  5. 5.
    CADWELL, P.R.B., WITTENBERG, B.A.: The oxygen dependency of mammalian tissues. Amer. J. Med.57 447–452 (1974)PubMedGoogle Scholar
  6. 6.
    CHAPMAN, N.D., GOLDSWORTHY, P.D., NYHUS, L.M., VOLWILER, W., HARKINS, H.N.: Studies in Isolated Organ Physiology: Bromsulphalein Clearance in Isolated perfused Bovine Liver. Surg.48 111–118 (1960)Google Scholar
  7. 7.
    CRAIG, A.B., jr.: Glucose, Lactate and Potassium Metabolism in the Isolated Perfused Rat Liver. Proc. Soc. exp. Biol. Med.121 281–286 (1966)PubMedGoogle Scholar
  8. 8.
    DAWKINS, M.J.R., JUDAH, J.D., REES, K.R.: Factor influencing the survival of liver cells during autolysis. J. Path. Bact.77 157 (1959)Google Scholar
  9. 9.
    DRAPANAS, T., ZEMEL, R., VANG, J.O.: Hemodynamics of the Isolated Perfused Pig liver: Metabolism According to Routes of Perfusion and Rates of Flow. Ann. Surg.164 522–537 (1966)PubMedGoogle Scholar
  10. 10.
    EISEMAN, B., KNIPE, P., McCOLL, H.A., ORLOFF, M.J.: Isolated liver perfusion for reducing blood ammonia. Arch. Surg.83 356–363 (1961)PubMedGoogle Scholar
  11. 11.
    EISEMAN, B., LIEM, D.S., RAFFUCCI, F.: Heterologous liver perfusion in treatment of hepatic failure. Ann. Surg.162 329–345 (1965)PubMedGoogle Scholar
  12. 12.
    ELMSLIE, R.G., PIROLA, R.C., CORONEOS, N., HAM, J.M.: Function of Liver after Isolated Cold Perfusion. Surg. Gynecol. Obstet.131 694–696 (1970)PubMedGoogle Scholar
  13. 13.
    GOLDBLATT, P.J., TRUMP, B.F., STOWELL, R.E.: Studies on necrosis of mouse liver in vitro. Amer. J. Path.47 183–208 (1965)PubMedGoogle Scholar
  14. 14.
    HARASZTI, A.: Mikroskopische und submikroskopische Untersuchungen der Eiweißtropfen enthaltenden Vakuolen der Leber. Exp. Path.3 82–93 (1969)Google Scholar
  15. 15.
    HARDISON, W.G.W., GREEN, E.A., NORMAN, J.C.: The viability and effect of flow upon function of the ex vivo perfused pig liver. J. Lab. Clin. Med.69 245–255 (1967)PubMedGoogle Scholar
  16. 16.
    HEMS, R., ROSS, B.D., BERRY, M.N., KREBS, H.A.: Glucogenesis in the perfused rat liver. Biochem. J.101 284–292 (1966)PubMedGoogle Scholar
  17. 17.
    HICKMAN, R., SAUNDERS, S.J., TERBLANCHE, J.: Liver Function in the Pig. Total Hepatic and Portal Flow Values in Vivo. S. Afr. Med. J.48 1197–1200 (1974)PubMedGoogle Scholar
  18. 18.
    NORMAN, J.C., COVELLI, W.H., HARDISON, W.G., McDERMOTT, W.V. jr.: Experimental studies related to clinical xenogeneic liver perfusions. Transplant.5 809–819 (1967)Google Scholar
  19. 19.
    RITCHIE, H.D., HARDCASTLE, J.D.: Isolated Organ Perfusion. Crosby Lockwood Staples London 1973Google Scholar
  20. 20.
    ROSCHLAU, G., KEMMER, C.: Experimentelle Untersuchungen zur Pinozytose und Phagozytose der Leberzelle. Virch. Arch. path. Anat.343 265–275, (1968)Google Scholar
  21. 21.
    SPALTEHOLZ, W.: Das "Durchsichtigmachen" als biologische Arbeitsmethode. In: Abderhalden, E.: Handbuch der biologischen Arbeitsmethoden IX/1. S. 409–432. Berlin-Wien: Urban und Schwarzenberg 1924Google Scholar
  22. 22.
    SPANNER, R.: Die Entwicklung der Darmzotten der Maus durch Knospung und Spaltung untersucht am Gefäßbaum. Gegenbaurs Morph. Jahrb. 67 S. 235–261 (1931)Google Scholar
  23. 23.
    TOTOVIC, V., LAGACE, R., LIE, T.S., BÜSSELBERG, E., YAMAMOTO, T.: Zur Ultrastruktur der Schweineleber bei 24-stündiger einfaeher hypothermer Konservierung. Res. exp. Med.158 129–151 (1972)Google Scholar
  24. 24.
    TYGSTRUP, N.: Perspectives in therapeutic pig liver perfusions. Scand. J. Gastroent.7 407–409 (1972)PubMedGoogle Scholar
  25. 25.
    VANG, J.O., DRAPANAS, T.: Metabolism of lactic acid and keto acids by the isolated perfused calf liver: Evaluation of Perfusion via Portal Vein and Hepatic Artery. Ann. Surg.163 545–552 (1966)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • M. Fischer
    • 1
  • Th. Wustrow
    • 1
  1. 1.Institut für Experimentelle Chirurgie und Chirurg. Klinik und Poliklinik r. d. IsarTechnischen Universität MünchenMünchen 80Germany

Personalised recommendations