Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Selective growth of epithelial-like clear cells from adult rat liver by short-term exposure to glucocorticoids in primary culture

  • 14 Accesses

  • 4 Citations


Isolated liver cells, which were prepared from adult rats by a trypsin-liver-perfusion technique, were treated with dexamethasone or hydrocortisone at a concentration of 7.7 × 10−6 M for 8 days in primary culture. The treated cultures displayed homogeneous population consisting of epithelial-like clear cells, while the untreated cultures displayed mixed population consisting of epithelial-like clear cells and fibroblast-like cells. The epithelial-like clear cells, which proliferated in the cultures treated with glucocorticoids for 8 days in primary culture, did not show any morphological changes following cultivation in glucocorticoid-free medium. After continuous glucocorticoid-treatment for more than 1 month, the treated cultures showed relatively low cell densities at confluence. The surface area of individual epithelial-like clear cells in the cultures treated with glucocorticoids for long periods of time was evidently greater than that in the cultures treated for only 8 days. The epithelial-like clear cells had glucose 6-phosphatase and tyrosine aminotransferase activities even though the levels of these enzymeactivities were very low compared with those in rat liver homogenates.

This is a preview of subscription content, log in to check access.


  1. 1.

    Berliner JA, Gerschenson LE (1975) The effects of a glucocorticoid on the cell surface of RLC-GAI cells. J Cell Physiol 86:523–531

  2. 2.

    Coon HG (1968) Clonal culture of differentiated rat liver cells. J Cell Biol 39:29a

  3. 3.

    Gaffney EV, Pigott D (1978) Hydrocortisone stimulation of human mammary epithelial cells. In Vitro 14:621–624

  4. 4.

    Gerschenson LE, Andersson M, Molson J, Okigaki T (1970) Tyrosine transaminase induction by dexamethasone in a new rat liver cell line. Science 20:859–861

  5. 5.

    Gerschenson LE, Okigaki T, Andersson M, Molson J, Davidson MB (1972) Fine structural and growth characteristics of cultured rat liver cells. Insulin effect. Exp Cell Res 71:49–58

  6. 6.

    Imai H (1977) Human embryonic liver cell in culture. A preliminary report. Okayama Iggakai Zasshi 89:237–244 (in Japanese)

  7. 7.

    Maca RD, Fry GL, Hoak JC (1978) The effects of glucocorticoids on cultured human endothelial cells. Br J Haematol 38:501–509

  8. 8.

    Miyazaki M (1977) Primary culture of adult rat liver cells. I. Preparation of isolated cells from trypsin-perfused liver of adult rat. Acta Med Okayama 31:351–360

  9. 9.

    Miyazaki M, Watanabe A, Shiraishi M, Hoshika T, Miyano K, Sato J (1978) Primary culture of adult rat liver cells. III. Hormonal effects on cytological and biochemical properties of primary cultured cells. Acta Med Okayama 32:85–96

  10. 10.

    Murison GL (1976) Growth of fetal rat liver cells in response to hydrocortisone. Exp Cell Res 100:439–443

  11. 11.

    Nakabayashi H (1981) Spontaneous neoplastic transformation in vitro of a diploid clone of rat liver cells under conditions of large inoculum size of cells at the transfer. Gann 72:377–387

  12. 12.

    Owens RB (1974) Glandular epithelial cells from mice: A method for selective cultivation. J Natl Cancer Inst 52:1375–1378

  13. 13.

    Ponec M, de Haas C, Bachra BN, Polano MK (1977) Effects of glucocorticosteroids on primary human skin fibroblasts. I. Inhibition of the proliferation of cultured primary human skin and mouse L929 fibroblasts. Arch Dermatol Res 259:117–123

  14. 14.

    Shiraishi M (1979) Malignant transformation of diploid rat liver cells treated with 3′-Me-DAB in tissue culture (in Japanese). Okayama Igakkai Zasshi 91:407–414

  15. 15.

    Tokiwa T, Nakabayashi H, Miyazaki M, Sato J (1979) Isolation and characterization of diploid clones from adult and newborn rat liver cell lines. In Vitro 15:393–400

  16. 16.

    Williams GM, Weisburger EK, Weisburger JH (1971) Isolation and long-term cell culture of epithelial-like cells from rat liver. Exp Cell Res 69:106–112

Download references

Author information

Correspondence to M. Miyazaki.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Miyazaki, M., Wahid, S., Miyano, K. et al. Selective growth of epithelial-like clear cells from adult rat liver by short-term exposure to glucocorticoids in primary culture. Res. Exp. Med. 181, 189–196 (1982). https://doi.org/10.1007/BF01851189

Download citation

Key words

  • Primary mass culture of adult rat liver cells
  • Glucocorticoids
  • Suppression of fibroblast-like cell growth
  • Epithelial-like clear cells
  • Liverspecific functions