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Selective growth of epithelial-like clear cells from adult rat liver by short-term exposure to glucocorticoids in primary culture

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Summary

Isolated liver cells, which were prepared from adult rats by a trypsin-liver-perfusion technique, were treated with dexamethasone or hydrocortisone at a concentration of 7.7 × 10−6 M for 8 days in primary culture. The treated cultures displayed homogeneous population consisting of epithelial-like clear cells, while the untreated cultures displayed mixed population consisting of epithelial-like clear cells and fibroblast-like cells. The epithelial-like clear cells, which proliferated in the cultures treated with glucocorticoids for 8 days in primary culture, did not show any morphological changes following cultivation in glucocorticoid-free medium. After continuous glucocorticoid-treatment for more than 1 month, the treated cultures showed relatively low cell densities at confluence. The surface area of individual epithelial-like clear cells in the cultures treated with glucocorticoids for long periods of time was evidently greater than that in the cultures treated for only 8 days. The epithelial-like clear cells had glucose 6-phosphatase and tyrosine aminotransferase activities even though the levels of these enzymeactivities were very low compared with those in rat liver homogenates.

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Correspondence to M. Miyazaki.

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Miyazaki, M., Wahid, S., Miyano, K. et al. Selective growth of epithelial-like clear cells from adult rat liver by short-term exposure to glucocorticoids in primary culture. Res. Exp. Med. 181, 189–196 (1982). https://doi.org/10.1007/BF01851189

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Key words

  • Primary mass culture of adult rat liver cells
  • Glucocorticoids
  • Suppression of fibroblast-like cell growth
  • Epithelial-like clear cells
  • Liverspecific functions