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Pathogenicity of quail's inclusion body hepatitis virus (avian adenovirus-1) for Japanese quails and broiler chicks


Quail (Coturnix coturnix japonica) and broiler (Gallus domesticus) chicks were inoculated experimentally with IBH virus (avian adenovirus-1) derived from quails to determine its pathogenicity. Quail chicks were inoculated by the intraperitoneal route at 3, 4, 5, 6 or 7 weeks of age. Lesions were encountered most frequently in the liver, kidneys and lungs. These included pale, swollen and mottled liver, swollen nephrotic kidneys, and congested and pneumonic lungs. The lesions were severe in birds inoculated at 5 weeks of age. Large basophilic intranuclear inclusion bodies were seen in hepatocytes and occasionally in the renal epithelium. The results showed that this isolate is pathogenic for quails above 3 weeks of age. Broiler chicks were inoculated at 4 weeks of age by the intraperitoneal route. The lesions produced in these chicks were similar to those of adenovirus-induced inclusion body hepatitis. Viral antigen was also demonstrated by dot-ELISA in suspensions of liver tissue from both quail and broiler chicks.

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amnio-allantoic fluid


avian adenovirus


days post inoculation

EID50 :

dose infective for 50% of embryos


enzyme-linked immunosorbent assay


inclusion body hepatitis


intranuclear inclusion bodies


normal allantoic fluid


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Singh, A., Oberoi, M.S. & Singh, B. Pathogenicity of quail's inclusion body hepatitis virus (avian adenovirus-1) for Japanese quails and broiler chicks. Vet Res Commun 19, 545–551 (1995).

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  • avian adenovirus
  • broiler chicks
  • inclusion body hepatitis
  • Japanese quails
  • pathogenicity
  • pathology