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Pathogenicity of quail's inclusion body hepatitis virus (avian adenovirus-1) for Japanese quails and broiler chicks

Abstract

Quail (Coturnix coturnix japonica) and broiler (Gallus domesticus) chicks were inoculated experimentally with IBH virus (avian adenovirus-1) derived from quails to determine its pathogenicity. Quail chicks were inoculated by the intraperitoneal route at 3, 4, 5, 6 or 7 weeks of age. Lesions were encountered most frequently in the liver, kidneys and lungs. These included pale, swollen and mottled liver, swollen nephrotic kidneys, and congested and pneumonic lungs. The lesions were severe in birds inoculated at 5 weeks of age. Large basophilic intranuclear inclusion bodies were seen in hepatocytes and occasionally in the renal epithelium. The results showed that this isolate is pathogenic for quails above 3 weeks of age. Broiler chicks were inoculated at 4 weeks of age by the intraperitoneal route. The lesions produced in these chicks were similar to those of adenovirus-induced inclusion body hepatitis. Viral antigen was also demonstrated by dot-ELISA in suspensions of liver tissue from both quail and broiler chicks.

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Abbreviations

AAF:

amnio-allantoic fluid

AAV:

avian adenovirus

DPI:

days post inoculation

EID50 :

dose infective for 50% of embryos

ELISA:

enzyme-linked immunosorbent assay

IBH:

inclusion body hepatitis

INIBs:

intranuclear inclusion bodies

NAF:

normal allantoic fluid

References

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Singh, A., Oberoi, M.S. & Singh, B. Pathogenicity of quail's inclusion body hepatitis virus (avian adenovirus-1) for Japanese quails and broiler chicks. Vet Res Commun 19, 545–551 (1995). https://doi.org/10.1007/BF01839342

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Keywords

  • avian adenovirus
  • broiler chicks
  • inclusion body hepatitis
  • Japanese quails
  • pathogenicity
  • pathology