Veterinary Research Communications

, Volume 19, Issue 6, pp 463–471 | Cite as

Preliminary findings of altered follicular activity in Holstein cows with coagulation factor XI deficiency

  • R. M. Liptrap
  • P. A. Gentry
  • M. L. Ross
  • E. Cummings


Factor XI (F XI) deficiency is an autosomal recessive coagulopathy found in Holstein cattle. Affected animals have a 50% greater prevalence of repeat breeding. Therefore, several parameters describing ovarian function were studied. Daily blood sampling revealed that progesterone concentrations were slower to decline from a peak at day 16 (p<0.01) to values less than 3 nmol/L in F XI-deficient cows (5.14±0.69 days (mean ± SD) versus 4.05±0.63 days in control animals), resulting in an oestrous cycle length of 24.7±2.1 days compared to 22.9±3.0 days, respectively. This was not due to an alteration in the availability of prostaglandin F (PGF) or oxytocin (OT) involved in luteolysis. No significant differences (p>0.05) were seen between normal (n=7) and F XI-deficient (n=7) cows in the peak values or the area under the curve for the pulse in 13,14-dihydro-15-keto PGF in response to OT challenge or in the parameters describing the pulse of ovarian OT secretion after PGF injection (n=7 for each) between days 12 and 14. Ovulatory follicular development was assessed by ultrasound monitoring and plasma 17β-oestradiol values at 8-h intervals after a luteolytic injection of cloprostenol (n=6 for each). Follicular diameter was smaller (p<0.05) and accompanied by lower peak oestradiol values near the time of ovulation in F XI-deficient cows. The results suggest that the oestrous cycle in F XI-deficient cows is characterized by a slower process of luteolysis that may be associated with smaller follicular development.


cow factor XI Holstein oestrous cycle ovary oxytocin progesterone prostaglandin F 



factor XI




prostaglandin F


13,14-dihydro-15-keto-prostaglandin F




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  1. Abraham, G.E., Swerdloff, R., Tulchinsky, D. and Odell, W.D., 1971. Radioimmunoassay of plasma progesterone.Journal of Clinical Endocrinology and Metabolism,32, 619–624PubMedGoogle Scholar
  2. Bolton-Maggs, P.H.B., Wan-Yin, B.Y., McCraw, A.H., Slark, J. and Kernoff, P.B.A., 1988. Inheritance and bleeding in factor XI deficiency.British Journal of Haematology,69, 521–528PubMedGoogle Scholar
  3. Brush, P.J., Anderson, P.H. and Gunning, R.F., 1987. Identification of factor XI deficiency in Holstein-Friesian cattle in Britain.Veterinary Record,121, 14–17PubMedGoogle Scholar
  4. Edqvist, L.E. and Johansson, E.D.B., 1972. Radioimmunoassay of oestrone and oestradiol in human and bovine peripheral plasma.Acta Endocrinologica,71, 716–730PubMedGoogle Scholar
  5. Fairclough, R.J. and Payne, E., 1975. Radioimmunoassay of 13,14-dihydro-15-keto-prostaglandin F in bovine peripheral plasma.Prostaglandins,10, 266–272PubMedGoogle Scholar
  6. Flint, A.P.F. and Sheldrick, E.L., 1983. Evidence for a systemic role for ovarian oxytocin in luteal regression in sheep.Journal of Reproduction and Fertility,67, 215–225PubMedGoogle Scholar
  7. Flint, A.P.F., Sheldrick, E.L., McCann, T.J. and Jones, D.S.C., 1990. Luteal oxytocin: characteristics and control of synchronous episodes of oxytocin and PGF secretion at luteolysis in ruminants.Domestic Animal Endocrinology,7, 111–124PubMedGoogle Scholar
  8. Fogwell, R.L., Cowley, J.L., Wortman, J.A., Ames, N.K. and Ireland, J.J., 1985. Luteal function in cows following destruction of ovarian follicles at midcycle.Theriogenology,23, 389–398Google Scholar
  9. Gentry, P.A. and Black, W.D., 1980. Prevalence and inheritance of factor XI (plasma thromboplastin antecedent) deficiency in cattle.Journal of Dairy Science,63, 616–620PubMedGoogle Scholar
  10. Gentry, P.A. and Ross, M.L., 1994. Coagulation factor XI deficiency in Holstein cattle: expression and distribution of factor XI activity.Canadian Journal of Veterinary Research,58, 242–247PubMedGoogle Scholar
  11. Gill, J.L. and Hafs, H.D., 1971. Analysis of repeated measurements of animals.Journal of Animal Science,32, 331–336Google Scholar
  12. Hooper, S.B. and Thorburn, G.D., 1987. Prostaglandin F and oxytocin release during persistence of the corpus luteum in sheep.Acta Endocrinologica,115, 469–477PubMedGoogle Scholar
  13. Hunter, R.H.F., 1980.Physiology and Technology of Reproduction in Female Domestic Animals, (Academic Press, London)Google Scholar
  14. Kindahl, H., Edqvist, L.E., Granström, E. and Bane, A., 1976. The release of prostaglandin F as reflected by 15-keto-13,14-dihydroprostaglandin F in the peripheral circulation during normal luteolysis in heifers.Prostaglandins,11, 871–878PubMedGoogle Scholar
  15. Kitchens, C.S., 1991. Factor XI: a review of its biochemistry and deficiency.Seminars in Thrombosis and Hemostasis,17, 55–72PubMedGoogle Scholar
  16. Larson, G.H., Williams, W.F., Gross, T.S., Russek-Cohen, E. and Manspeaker, J.E., 1991. Systemic 13,14-dihydro-15-keto-prostaglandin F (PGFM) response to exogenous estradiol by luteal heifers on days 14 and 19 of the estrous cycle and on day 19 of pregnancy.Animal Reproduction Science,25, 31–39Google Scholar
  17. Lehwald van Voorst, N.A.M., 1993.Protein profile of bovine follicular fluid and the synthesis of fibronectin by bovine granulosa cells, (MSc thesis, University of Guelph)Google Scholar
  18. Leiba, H., Ramot, B. and Many, R., 1965. Heredity and coagulation studies in ten families with factor XI (plasma thromboplastin antecedent) deficiency.British Journal of Haematology,11, 654–665PubMedGoogle Scholar
  19. Rapaport, S.I., Proctor, R.R., Patch, M.J. and Yetta, M., 1961. The mode of inheritance of PTA deficiency: evidence for the existence of major PTA deficiency and minor PTA deficiency.Blood,18, 149–165PubMedGoogle Scholar
  20. Razni, M.V., Sinha, D., Seaman, F., Lewis, J.H., Spers, J.A. and Walsh, P.N., 1985. Comparison of bleeding tendency, factor XI coagulation activity, and factor XI antigen in 25 factor XI-deficient kindred.Blood,65, 719–724PubMedGoogle Scholar
  21. Seligsohn, U., 1978. High gene frequency of factor XI (PTA) deficiency in Ashkenazi Jews.Blood,51, 1223–1228PubMedGoogle Scholar
  22. Seligsohn, U., 1993. Factor XI deficiency.Thrombosis and Haemostasis,70, 68–71PubMedGoogle Scholar
  23. Silvia, W.J. and Raw, R.E., 1993. Regulation of pulsatile secretion of prostaglandin F from the ovine uterus by ovarian steroids.Journal of Reproduction and Fertility,98, 341–347PubMedGoogle Scholar
  24. Silvia, W.J., Lewis, G.S., McCracken, J.A., Thatcher, W.W. and Wilson, L. Jr., 1991. Hormonal regulation of uterine secretion of prostaglandin F secretion during luteolysis in ruminants.Biology of Reproduction,45, 655–663PubMedGoogle Scholar
  25. Steel, R.G.D. and Torrie, J.H., 1980.Principles and Procedures of Statistics, (McGraw-Hill, New York)Google Scholar
  26. Thatcher, W.W., Terqui, M., Thimonier, J. and Mauleon, P., 1986. Effect of estradiol-17β on peripheral plasma concentration of 15-keto-13,14-dihydro PGF and luteolysis in cyclic cattle.Prostaglandins,31, 745–756PubMedGoogle Scholar
  27. Veldhuis, J.D. and Johnson, M.L., 1986. Cluster analysis: a simple, robust algorithm for endocrine pulse detection.American Journal of Physiology,250, E486-E493PubMedGoogle Scholar
  28. Vighio, G.H. and Liptrap, R.M., 1986. Plasma concentrations of oxytocin, prostaglandin and ovarian steroids during spontaneous luteolysis in the cow.Domestic Animal Endocrinology,3, 209–215Google Scholar
  29. Yamada, M. and Gentry, P.A., 1994. The hemostatic profile of equine follicular fluid.Thrombosis Research,77, 45–54Google Scholar
  30. Yamada, M. and Gentry, P.A., 1995. Hemostatic profile of bovine ovarian follicular fluid.Canadian Journal of Physiology and Pharmacology,73, 624–629PubMedGoogle Scholar
  31. Zarco, L., Stabenfeldt, G.H., Quirke, J.F., Kindahl, H. and Bradford, G.E., 1988. Release of prostaglandin-F2α and timing of events associated with luteolysis in ewes with oestrous cycles of different lengths.Journal of Reproduction and Fertility,83, 517–526PubMedGoogle Scholar
  32. Zhang, J., Weston, P.G. and Hixon, J.E., 1991. Influence of estradiol on the secretion of oxytocin and prostaglandin F during luteolysis in the ewe.Biology of Reproduction,45, 395–403PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers bv 1995

Authors and Affiliations

  • R. M. Liptrap
    • 1
  • P. A. Gentry
    • 1
  • M. L. Ross
    • 1
  • E. Cummings
    • 1
  1. 1.Department of Biomedical SciencesUniversity of GuelphGuelphCanada

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