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Veterinary Research Communications

, Volume 16, Issue 4, pp 265–272 | Cite as

Preparation of latex sensitized with rabbit IgG antibody for slide reversed passive agglutination

  • S. Yamamoto
  • K. Tagata
  • Y. Ishikawa
  • H. Fujise
  • H. Nagahata
  • M. Yamada
  • T. Sakano
  • M. Morimatsu
  • M. Naiki
Immunology

Abstract

A method is described for preparing latex particles sensitized with IgG antibody (IgG-sensitized latex) applicable to the slide reversed passive agglutination (RPLA) test. Soap-free latex (latex) was sensitized with IgG which had been isolated from rabbit anti-bovine lactoferrin serum using protein A Sepharose CL 4B. Unadsorbed protein-binding sites on the surface of latex were blocked with bovine serum albumin (BSA). IgG-sensitized latex that gave better agglutination in RPLA could be selectively obtained by centrifugation at 19 900g for 15 min in 0.01 mol/L glycine buffer (pH 7.3; specific gravity 1.042) containing 3% NaCl, 5% saccharose and 2% choline chloride. By dispersing this IgG-sensitized latex in 0.01 mol/L glycine buffer (pH 7.3) containing 1–2% BSA, a uniformly suspended, highly reactive, readily agglutinable preparation was obtained.

Keywords

agglutination IgG-sensitized latex lactoferrin reversed passive latex agglutination RPLA 

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Copyright information

© Kluwer Academic Publishers bv 1992

Authors and Affiliations

  • S. Yamamoto
    • 1
  • K. Tagata
    • 1
  • Y. Ishikawa
    • 1
  • H. Fujise
    • 1
  • H. Nagahata
    • 2
  • M. Yamada
    • 3
  • T. Sakano
    • 4
  • M. Morimatsu
    • 5
  • M. Naiki
    • 5
  1. 1.Laboratory of Immunology, Faculty of Environmental HealthAzabu UniversitySagamihara, KanagawaJapan
  2. 2.Department of Animal Hygiene, School of Veterinary MedicineRakuno Gakuen UniversityEbetsu, HokkaidoJapan
  3. 3.Department of Bioresource DevelopmentShobara, HiroshimaJapan
  4. 4.Zen-noh Institute of Animal HealthSakura, ChibaJapan
  5. 5.Department of Biochemistry, Faculty of Veterinary MedicineHokkaido UniversitySapporo, HokkaidoJapan

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