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Veterinary Research Communications

, Volume 19, Issue 3, pp 179–184 | Cite as

Training-induced modifications in some biochemical defences against free radicals in equine erythrocytes

  • L. Avellini
  • M. Silvestrelli
  • A. Gaiti
Biochemistry

Abstract

Oxidative stress develops when the generation of free radicals exceeds the antioxidant capacity of cells or extracellular fluids. It can also occur as a result of physical exercise, and the pathogenesis of exercise-induced myopathies and haemolysis in horses may be related to changes in lipid peroxidation caused by free radicals. Cells have developed biochemical protection against oxidative stress and, as tissues seem to increase their antioxidant defences under chronic activation, training may be one of the ways of increasing antioxidant defences. Accordingly, we tested some enzymatic antioxidant activities as well as nonenzymatic antioxidants in horses undergoing special training. The results indicated a decrease in both chemical and biochemical defences against free radicals during training. It was deduced that the horses' diet may have been unable to provide the increased need for antioxidant defences resulting from training.

Keywords

α-toxopherol antioxidant erythrocytes free radicals glutathione peroxidase horse oxidative stress training vitamin E 

Abbreviations

DPH

1,6-diphenyl-1,3,5-hexatriene

EU

enzymic unit

GSH-Px

glutathione peroxidase

P

degree of fluorescence polarization

Phospholipid P

phosphorous content in phospholipids

RBC

red blood cell

t-ButOOH

t-butylhydroperoxide

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Copyright information

© Kluwer Academic Publishers bv 1995

Authors and Affiliations

  • L. Avellini
    • 1
  • M. Silvestrelli
    • 2
  • A. Gaiti
    • 1
  1. 1.Istituto di Biochimica e Chimica MedicaUniversità di PerugiaPerugiaItaly
  2. 2.Centro del Cavallo Sportivo, Facoltà di Medicina VeterinariaUniversità di PerugiaPerugiaItaly

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