Thein vitro synergistic effects of combinations of EDTA-tromethamine and six antimicrobial agents (ampicillin, chloramphenicol, oxytetracycline, streptomycin, nalidixic acid and sulphadimethoxine) on clinically isolated strains ofPseudomonas aeruginosa, Proteus mirabilis andEscherichia coli were investigated. The antibacterial activity was assessed from the minimal inhibitory concentration for the antibiotics alone or in combination with EDTA-tromethamine. EDTA-tromethamine potentiated the antibacterial activity of ampicillin, chloramphenicol, oxytetracycline and streptomycin up to four-fold. There were no significant or consistent synergistic effects with nalidixic acid or sulphadimethoxine.
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minimal inhibitory concentration
Ashworth, C.D. and Nelson, D.R., 1990. Antimicrobial potentiation of irrigation solutions containing tris-(hydroxymethyl) aminomethane-EDTA.Journal of the American Veterinary Medical Association,197, 1513–1514
Bjorling, D.E. and Wooley, R.E., 1982. EDTA-tromethamine lavage as an adjunct treatment for multiple fistulas in a dog.Journal of the American Veterinary Medical Association,181, 596–597
Blue, J.L., Wooley, R.E. and Eagon, R.G., 1974. Treatment of experimentally inducedPseudomonas aeruginosa otitis externa in the dog by lavage with ethylenediaminetetracetate-Tris (hydroxymethyl) aminomethane lysozyme.American Journal of Veterinary Research,35, 1221–1223
Farca, A.M., Nebbia, P. and Dacasto, M., 1992. Effetto dell'associazione di diversi chemioantibiotici sulla loro attività antibatterica in vitro.Obiettivi & Documenti Veterinari,3, 37–41
Gerberick, G.F. and Castric, P.A., 1980.In vitro susceptibility ofPseudomonas aeruginosa to carbenicillin, glycine and ethylenediaminetetraacetic acid combinations.Antimicrobial Agents and Chemotherapy,17, 732–735
Isaksson, I., Hanberger, H., Maller, R., Nilsson, L.E. and Nilsson, M., 1991. Synergic post-antibiotic effect of amikacin in combination with β-lactam antibiotics on Gram-negative bacteria.Journal of Antimicrobial Chemotherapy,28, 25–34
Joklik, W.K., Willett, H.P. and Amos, D.B., 1980.Zinsser Microbiology, 17th edn, (Appleton-Century-Crofts, New York), 234–277
MacFaddin, J.F., 1980.Biochemical Tests for Identification of Medical Bacteria, 2nd edn, (Baltimore, Williams & Wilkins)
Pearman, J.W., Bailey, M. and Harper, W.E., 1988. Comparison of the efficacy of ‘Trisdine’ and kanamycin-colistin bladder installations in reducing bacteriuria during intermittent catheterization of patients with acute spinal cord trauma.British Journal of Urology,62, 140–144
Wooley, R.E., Schall, W.D., Eagon, R.G. and Scott, A.A.S., 1974. Efficacy of EDTA-Tris-lysozyme lavage in the treatment of experimentally inducedPseudomonas aeruginosa cystitis in the dog.American Journal of Veterinary Research,35, 227–229
Wooley, R.E., Bermann, A.P. and Shotts, E.B. Jr., 1979. Antibiotic-tromethamine-EDTA lavage for the treatment of bacterial rhinitis in a dog.Journal of the American Veterinary Medical Association,175, 817–818
Wooley, R.E., Gilbert, M.S. and Shotts, E.B. Jr., 1981. Inhibitory effects of combinations of oxytetracycline, dimethyl sulfoxide, and EDTA-tromethamine onEscherichia coli.American Journal of Veterinary Research,42, 2010–2013
Wooley, R.E., Jones, M.S., Gilbert, J.P. and Shotts, E.B. Jr., 1983.In vitro action of combinations of antimicrobial agents and EDTA-tromethamine onEscherichia coli.American Journal of Veterinary Research,44, 1154–1158
Wooley, R.E., Jones, M.S., Gilbert, J.P. and Shotts, E.B. Jr., 1984.In vitro action of combinations of antimicrobial agents with EDTA-tromethamine onProteus vulgaris of canine origin.American Journal of Veterinary Research,45, 1451–1454
Youngquist, R.S., 1975.Pseudomonas metritis in a mare.Veterinary Medicine Small Animal Clinician,70, 340–342
Yuan, D. and Shen, V., 1975. Stability of ribosomal and transfer ribonucleic acid inEscherichia coli B/V after treatment with ethylenedinitrilotetraacetic acid and rifampin.Journal of Bacteriology,122, 425–432
Part of this paper was communicated at the XLV Congress of the Italian Society of Veterinary Sciences, Palermo, 25–28 September 1991
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Farca, A.M., Nebbia, P. & Re, G. Potentiation of thein vitro activity of some antimicrobial agents against selected Gram-negative bacteria by EDTA-tromethamine. Vet Res Commun 17, 77–84 (1993). https://doi.org/10.1007/BF01839235
- ethylenediaminetetraacetic acid
- minimal inhibitory concentration