Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Potentiation of thein vitro activity of some antimicrobial agents against selected Gram-negative bacteria by EDTA-tromethamine

Abstract

Thein vitro synergistic effects of combinations of EDTA-tromethamine and six antimicrobial agents (ampicillin, chloramphenicol, oxytetracycline, streptomycin, nalidixic acid and sulphadimethoxine) on clinically isolated strains ofPseudomonas aeruginosa, Proteus mirabilis andEscherichia coli were investigated. The antibacterial activity was assessed from the minimal inhibitory concentration for the antibiotics alone or in combination with EDTA-tromethamine. EDTA-tromethamine potentiated the antibacterial activity of ampicillin, chloramphenicol, oxytetracycline and streptomycin up to four-fold. There were no significant or consistent synergistic effects with nalidixic acid or sulphadimethoxine.

This is a preview of subscription content, log in to check access.

Abbreviations

cfu:

colony-forming units

EDTA:

ethylenediaminetetraacetic acid

MIC:

minimal inhibitory concentration

tromethamine:

tris(hydroxymethyl)aminomethane

References

  1. Ashworth, C.D. and Nelson, D.R., 1990. Antimicrobial potentiation of irrigation solutions containing tris-(hydroxymethyl) aminomethane-EDTA.Journal of the American Veterinary Medical Association,197, 1513–1514

  2. Bjorling, D.E. and Wooley, R.E., 1982. EDTA-tromethamine lavage as an adjunct treatment for multiple fistulas in a dog.Journal of the American Veterinary Medical Association,181, 596–597

  3. Blue, J.L., Wooley, R.E. and Eagon, R.G., 1974. Treatment of experimentally inducedPseudomonas aeruginosa otitis externa in the dog by lavage with ethylenediaminetetracetate-Tris (hydroxymethyl) aminomethane lysozyme.American Journal of Veterinary Research,35, 1221–1223

  4. Farca, A.M., Nebbia, P. and Dacasto, M., 1992. Effetto dell'associazione di diversi chemioantibiotici sulla loro attività antibatterica in vitro.Obiettivi & Documenti Veterinari,3, 37–41

  5. Gerberick, G.F. and Castric, P.A., 1980.In vitro susceptibility ofPseudomonas aeruginosa to carbenicillin, glycine and ethylenediaminetetraacetic acid combinations.Antimicrobial Agents and Chemotherapy,17, 732–735

  6. Isaksson, I., Hanberger, H., Maller, R., Nilsson, L.E. and Nilsson, M., 1991. Synergic post-antibiotic effect of amikacin in combination with β-lactam antibiotics on Gram-negative bacteria.Journal of Antimicrobial Chemotherapy,28, 25–34

  7. Joklik, W.K., Willett, H.P. and Amos, D.B., 1980.Zinsser Microbiology, 17th edn, (Appleton-Century-Crofts, New York), 234–277

  8. MacFaddin, J.F., 1980.Biochemical Tests for Identification of Medical Bacteria, 2nd edn, (Baltimore, Williams & Wilkins)

  9. Pearman, J.W., Bailey, M. and Harper, W.E., 1988. Comparison of the efficacy of ‘Trisdine’ and kanamycin-colistin bladder installations in reducing bacteriuria during intermittent catheterization of patients with acute spinal cord trauma.British Journal of Urology,62, 140–144

  10. Wooley, R.E., Schall, W.D., Eagon, R.G. and Scott, A.A.S., 1974. Efficacy of EDTA-Tris-lysozyme lavage in the treatment of experimentally inducedPseudomonas aeruginosa cystitis in the dog.American Journal of Veterinary Research,35, 227–229

  11. Wooley, R.E., Bermann, A.P. and Shotts, E.B. Jr., 1979. Antibiotic-tromethamine-EDTA lavage for the treatment of bacterial rhinitis in a dog.Journal of the American Veterinary Medical Association,175, 817–818

  12. Wooley, R.E., Gilbert, M.S. and Shotts, E.B. Jr., 1981. Inhibitory effects of combinations of oxytetracycline, dimethyl sulfoxide, and EDTA-tromethamine onEscherichia coli.American Journal of Veterinary Research,42, 2010–2013

  13. Wooley, R.E., Jones, M.S., Gilbert, J.P. and Shotts, E.B. Jr., 1983.In vitro action of combinations of antimicrobial agents and EDTA-tromethamine onEscherichia coli.American Journal of Veterinary Research,44, 1154–1158

  14. Wooley, R.E., Jones, M.S., Gilbert, J.P. and Shotts, E.B. Jr., 1984.In vitro action of combinations of antimicrobial agents with EDTA-tromethamine onProteus vulgaris of canine origin.American Journal of Veterinary Research,45, 1451–1454

  15. Youngquist, R.S., 1975.Pseudomonas metritis in a mare.Veterinary Medicine Small Animal Clinician,70, 340–342

  16. Yuan, D. and Shen, V., 1975. Stability of ribosomal and transfer ribonucleic acid inEscherichia coli B/V after treatment with ethylenedinitrilotetraacetic acid and rifampin.Journal of Bacteriology,122, 425–432

Download references

Author information

Additional information

Part of this paper was communicated at the XLV Congress of the Italian Society of Veterinary Sciences, Palermo, 25–28 September 1991

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Farca, A.M., Nebbia, P. & Re, G. Potentiation of thein vitro activity of some antimicrobial agents against selected Gram-negative bacteria by EDTA-tromethamine. Vet Res Commun 17, 77–84 (1993). https://doi.org/10.1007/BF01839235

Download citation

Keywords

  • antibiotics
  • bacteria
  • Gram-negative
  • EDTA
  • ethylenediaminetetraacetic acid
  • minimal inhibitory concentration
  • potentiation
  • tromethamine