Veterinary Research Communications

, Volume 17, Issue 4, pp 283–294 | Cite as

The effects of naturally deoxynivalenol-contaminated oats on the clinical condition, blood parameters, performance and carcass composition of growing pigs

  • B. Bergsjø
  • W. Langseth
  • I. Nafstad
  • J. Høgset Jansen
  • H. J. S. Larsen
Pathology

Abstract

A feeding trial with naturally deoxynivalenol (DON)-contaminated oats included in feed mixtures at graded levels was conducted in growing pigs. The DON concentrations were 0, 0.7, 1.7, and 3.5 mg/kg of complete feed mixture givenad libitum to different groups. The data recorded were feed consumption, body weight gain, slaughter weight, biochemical and haematological data including serum immunoglobulin A, clinical condition and post-mortem pathology including histopathology.

Significantly decreasing body weight gain throughout the experimental period, decreased slaughter weight and reduced feed utilization efficiency were observed for the group fed a diet containing 3.5 mg/kg of DON. At the same DON concentration, there were increased liver weights and decreased concentrations of serum protein and albumin, and a temporary fall in packed blood cell volume, serum calcium and serum phosphorus. For the groups fed diets containing 1.7 and 3.5 mg/kg of DON, a statistically significant, dose-related decrease in daily feed consumption was observed. No other effects on haematological, biochemical or immunological parameters were recorded. The carcass quality was not affected in any group.

It was concluded that significant effects in growing pigs may be observed at a dietary DON concentration of 1.7 mg/kg, originating from naturally contaminated oats included in a diet that was otherwise adequate and contained only minor traces of other mycotoxins.

Keywords

deoxynivalenol mycotoxin oats pathology pig trichothecenes 

Abbreviations

ALAT

alanine aminotransferase

ASAT

aspartate aminotransferase

CFU

colony-forming units

DON

deoxynivalenol

3-ac-DON

3-acetyl-deoxynivalenol

F.U.

feed unit

FUS-X

fusarenon-X

HPLC

high-pressure liquid chromatography

IgA

immunoglobulin A

NFSA

nutrient-free sporulation agar

NIV

nivalenol

PDA

potato dextrose agar

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References

  1. Beasly, V.R., 1989. In:Trichothecene Mycotoxicosis: Pathophysiologic Effects, vol. I, (CRC Press, Boca Raton, FL)Google Scholar
  2. Bergsjø, B., Matre, T. and Nafstad, I., 1992. Effects of diets with graded levels of deoxynivalenol on performance in growing pigs.Journal of Veterinary Medicine,A39, 752–758Google Scholar
  3. Chavez, E.R., 1984. Vomitoxin-contaminated wheat in pig diets: Pregnant and lactating gilts and weaners.Canadian Journal of Animal Science,64, 717–723Google Scholar
  4. Chelkowski, J., 1989. In:Fusarium, Mycotoxins, Taxonomy and Pathogenicity. Topics in Secondary Metabolites, vol. 2, (Elsevier, Amsterdam)Google Scholar
  5. Friend, D.H., Trenholm, H.L., Thompson, B.K., Fiser, P.S. and Hartin, K.E., 1986. Effect of feeding diets containing deoxynivalenol (vomitoxin)-contaminated wheat or corn on the feed consumption, weight gain, organ weight and sexual development of male and female pigs.Canadian Journal of Animal Science,66, 765–775Google Scholar
  6. Forsyth, D.M., Yoshizawa, T., Morooka, N. and Tuite, J., 1977. Emetic and refusal activity of deoxynivalenol to swine.Applied and Environmental Microbiology,34, 547–552PubMedGoogle Scholar
  7. Hansson, I., Lundstrøm, K. and Bjärstorp, G., 1986. Simultaneous grading and meat quality measurements by the Hennesy Grading System.The 32nd European Meeting of Meat Research Workers, Ghent, 1986Google Scholar
  8. Langseth, W. and Clasen, P.E., 1992. Automation of a clean-up procedure for determination of trichothecenes in cereals using a charcoal-alumina column.Journal of Chromatography,603, 290–293PubMedGoogle Scholar
  9. Langseth, W., Gullord, M. and Høie, R., 1992. The influence of climatic conditions on formation of trichothecenes in different oat cultivars in Norway. AtThe VIII International IUPAC Symposium on Mycotoxins and Phycotoxins, Mexico, November 1992 [not published]Google Scholar
  10. Langseth, W., Ellingsen, Y., Nymoen, U. and Økland, E.M., 1989. High-performance liquid chromatographic determination of zearalenone and ochratoxin A in cereals and feed.Journal of Chromatography,478, 269–274Google Scholar
  11. Liao, L.L., Grollman, A.P. and Horrwitz, S.B., 1976. Mechanism of the action of the 12,13-epoxytrichothecene anguidine: an inhibitor of protein synthesis.Biochimica et Biophysica Acta,452(2, 273–284Google Scholar
  12. Lun, A.K., Young, L.G., and Lumsden, J.H., 1985. The effects of vomitoxin and feed intake on the performance and blood characteristics of young pigs.Journal of Animal Science,61, 1178–1185PubMedGoogle Scholar
  13. Mancini, G., Garbonara, A. and Heremans, J.F., 1965. Immunochemical quantitation of antigens by single radial immunodiffusion.Immunochemistry,2, 235–254PubMedGoogle Scholar
  14. Nelson, P.E., Tousson, T.A. and Marasas, W.F.O., 1983. In:Fusarium Species: An Illustrated Manual for Identification, (The Pennsylvania University Press, University Park of London)Google Scholar
  15. Pestka, J.J., Moorman, M.A. and Warner, R.L., 1989. Dysregulation of IgA production and IgA nephropathy induced by the trichothecene vomitoxin.Food and Chemical Toxicology,27, 361–368PubMedGoogle Scholar
  16. SAS, 1985.SAS Users Guide: Statistics, (SAS Institute Inc., Cary, NC)Google Scholar
  17. Schuh, M., Leibetseder, J. and Glawischnig, E., 1982. Chronic effects of different levels of deoxynivalenon (vomitoxin) on weight gain, feed consumption, blood parameters, pathological as well as histopathological changes in fattening pigs.V International IUPAC Symposium on Mycotoxins and Phycotoxins, Vienna, 1982Google Scholar
  18. Stenwig, H., Langseth, W. and Sandli, D., 1992. The occurrence of trichothecenes and moulds in samples of barley and oats from Norway.Mycotoxin Research, 34th European Seminar on Fusarium, Poland, Sept., 1992Google Scholar
  19. Sundheim, L., Nagayama, S., Kawamura, O., Tanaka, T., Brodal, G. and Ueno, O., 1988. Trichothecenes and zearalenone in Norwegian barley and wheat.Norwegian Journal of Agricultural Sciences,2, 49–59Google Scholar
  20. Ueno, Y., 1983. In:Trichothecenes, Chemical, Biological and Toxicological Aspects, Development in Food Science, vol. 4, (Elsevier, Amsterdam)Google Scholar
  21. Wei, C.M. and Maclaughlin, C.S., 1974. Structure-function relationship in the 12,13-epoxytrichothecenes (novel inhibitors of protein synthesis).Biochemical and Biophysical Research Communication,57, 838Google Scholar
  22. Young, L.G., McGirr, L., Valli, V.E., Lumsden, J.H. and Lun, A., 1983. Vomitoxin in corn fed to young pigs.Journal of Animal Science,57, 655–664PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers bv 1993

Authors and Affiliations

  • B. Bergsjø
    • 1
  • W. Langseth
    • 1
  • I. Nafstad
    • 2
  • J. Høgset Jansen
    • 3
  • H. J. S. Larsen
    • 4
  1. 1.Department of Toxicology and ChemistryNational Veterinary InstituteOslo 1Norway
  2. 2.Department of Pharmacology and ToxicologyNorwegian College of Veterinary MedicineOslo 1Norway
  3. 3.Department of PathologyNorwegian College of Veterinary MedicineOslo 1Norway
  4. 4.Department of Microbiology and ImmunologyNorwegian College of Veterinary MedicineOslo 1Norway

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